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The figure below is the diagrammatic representation of a typical agarose gel electrophoresis.

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Important Questions on Biotechnology - Principles and Processes

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Amplification of gene of interest by using PCR for 30 cycles may go upto
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The thermostable enzymes, 'Taq’ and 'Pfu', isolated from thermophilic bacteria are:
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Read statements 'A' and 'B' and choose the correct option.

Statement A : Agrobacterium tumefaciens causes crown gall in dicots

Statement B : Agrobacterium tumefaciens enters the host through wound and injuries in certain plants.

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The correct order of steps in Polymerase Chain Reaction (PCR) is:
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While constructing the first recombinant DNA molecule, Stanley Cohen and Herbert Boyer isolated plasmid from A while B acted as host for the recombinant plasmid.
Select the option which gives correct answer for blanks in above statements.
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Select the option which gives correct name for the restriction enzyme isolated from Haemophilus influenzae.
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Extraction of DNA fragments from agarose gel is known as
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If we want to recover more copies of target (desired) DNA then it should be cloned in a vector having