Embibe Experts Solutions for Chapter: Biotechnology - Principles and Processes, Exercise 3: Level 3

Choose the wrong statement from the following:

Match the following.

Consider the linear double-stranded DNA shown below. The restriction enzyme sites and the lengths demarcated are shown. This DNA is completely digested with both $\mathrm{EcoRI}$ and $\mathrm{BamHI}$ restriction enzymes. If the Product is analyzed by gel electrophoresis, how many distinct bands would be observed?

A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be

Four mutant strains of bacteria $(1-4)$ all require substance $S$ to grow (each strain is blocked at one step in the $S-$biosynthesis pathway). Four plates were prepared with minimal medium and a trace of substance $S,$ to allow a small amount of growth of mutant cells. On plate $A$, mutant cells of strain $1$ were spread over entire surface of the agar to form a thin lawn of bacteria. On plate $B$, the lawn was composed of mutant cells of strain $2,$ and so
on. On each plate, cells of each of the four mutant types were inoculated over the lawn, as indicated in the figure by the circles. Dark circles indicate excellent growth. A strain blocked at a later step in the $S$ substance metabolic pathway accumulates intermediates that can 'feed' a strain blocked at an earlier step.

What is the order of genes $(1-4)$ in the metabolic pathway for synthesis of substance $S ?$

Match the tissues/molecules mentioned in Column I with those of the degrading enzymes mentioned in Column II and select the correct option.

One billion copies of DNA are obtained by PCR technique after _____ cycles.

The basic procedure involved in the synthesis of recombinant $\mathrm{DNA}$ molecule is depicted below.

The mistake in the procedure is: