Embibe Experts Solutions for Chapter: Biotechnology - Principles and Processes, Exercise 1: NEET - 17th July 2022

Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A): Polymerase chain reaction is used in DNA amplification.
Reason (R): The ampicillin-resistant gene is used as a selectable marker to check the transformation.
In light of the above statements, choose the correct answer from the options given below :

Which one of the following statement is not true regarding gel electrophoresis technique?

Transposons can be used during which one of the following?

In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme?

Given below are two statements:

Statement $\mathrm{I}$: Restriction endonucleases recognise specific sequence to cut DNA known as palindromic nucleotide sequence.

Statement $\mathrm{II}$: Restriction endonucleases cut the DNA strand a little away from the centre of the palindromic site.

In the light of the above statements, choose the most appropriate answer from the options given below:

Which of the following is not a desirable feature of a cloning vector?

Plasmid $\mathrm{pBR}322$ has PstI restriction enzyme site within gene ${\mathrm{amp}}^{\mathrm{R}}$ that confers ampicillin resistance. If this enzyme is used for inserting a gene for $\mathrm{\beta }$-galactoside production and the recombinant plasmid is inserted in an E. coli strain

In Recombinant $\mathrm{DNA}$ technology antibiotics are used:

*Note: There was ambiguity in options in the original paper, hence the options are changed.