Match the words of Group A with those of group B to make meaningful pairs: Group A Group B Polyacrylamide Agrobacterium tumefaciens Southern blotting Thermocycler Plasmid Protein electrophoresis Agarose gel Denaturation Cos Taq polymerase Tumor inducing plasmid Cloning vehicle Recombinant DNA Molecular marker DNA coated tungsten particles Nucleic acid electrophoresis Insertional inactivation Chimeric DNA Breaking of interchain hydrogen bonds Particle gun Equipment for PCR amplification Screening of clones Ampicillin resistance gene Blotting of DNA Thermophilus aquaticus Cohesive site

Polyacrylamide- Protein electrophoresis- Polyacrylamide Gel Electrophoresis is an analytical method used to separate protein mixture based on their size. The technique is based upon the principle that a charged molecule will migrate in an electric field towards an electrode with the opposite sign. Southern blotting- Blotting of DNA- Southern blotting is a technique used to detect a specific DNA sequence in a blood or tissue sample. A restriction endonuclease is used to cut a sample of DNA into fragments that are separated using gel electrophoresis. Plasmid- Cloning vector- Plasmids are cloning vectors that are maintained in cells as autonomously replicating circular double-stranded DNA molecules. Agarose gel-Nucleic acid electrophoresis- Agarose gels are made by dissolving agarose in the buffer to form a porous and resilient matrix for nucleic acid separation. Cos- Cohesive site- The cos site represents the junction between two genomes in a concatemer and serves as the packaging initiation site. Tumor-inducing plasmid- Agrobacterium tumefaciens - A plant pathogenic bacterium that contains a plasmid, the tumor-inducing or Ti plasmid, a segment of which, called T-DNA, integrates into the host plant chromosomes causing a cancerous proliferation. Recombinant DNA- Chimeric DNA- Recombinant DNA molecules are called chimeric DNA, because they can be made of the base sequences from two different species, like the mythical chimera. Proteins that expresses from recombinant DNA within living cells are termed recombinant proteins. DNA coated tungsten particles- Particle gun- In plants, to get foreign DNA into chloroplasts as well as genomic DNA, a gene gun is employed that fires small DNA-coated particles (typically gold or tungsten particles because they are dense) into target cells. Insertional inactivation- Molecular marker- Insertional inactivation is a technique used in recombinant DNA technology to identify transformed cells that took up recombinant plasmids. Ligation of the DNA insert into the polylinker site consistently causes disruption of the selectable marker gene. This process is known as insertional inactivation. Breaking of interchain hydrogen bonds- Denaturation- The denaturation process involves the breaking of many of the weak linkages, or bonds, for example, hydrogen bonds. These bonds are often present within a protein molecule that are responsible for the highly ordered quaternary structure of polypeptides in their native state. Equipment for PCR amplification- Thermocycler- The Thermocycler is a laboratory apparatus used to amplify segments of DNA via the Polymerase Chain Reaction (PCR). The device has a thermal block with holes where tubes holding the PCR reaction mixtures can be inserted. Ampicillin resistance gene- Screening of clones- Adding an antibiotic resistance gene to the plasmid vector allows a scientist to easily detect plasmid-containing bacteria when the cells are grown on selective media and provides those bacteria with pressure to retain the foreign plasmid. Thermophilus aquaticus - Taq polymerase- This polymerase is frequently used in PCR to amplify target DNA . Taq polymerase has thermostability as it is isolated from a bacterium Thermophilus aquaticus that lives in hot springs. Hence, the correctly matched pairs are as follows: Group A Group B Polyacrylamide Protein electrophoresis Southern blotting Blotting of DNA Plasmid Cloning vector Agarose gel Nucleic acid electrophoresis Cos Cohesive site Tumor inducing plasmid Agrobacterium tumefaciens Recombinant DNA Chimeric DNA DNA coated tungsten particles Particle gun Insertional inactivation Molecular marker Breaking of interchain hydrogen bonds Denaturation Equipment for PCR amplification Thermocycler Ampicillin resistance gene Screening of clones Thermophilus aquaticus Taq polymerase

Odisha Board Solutions for Exercise 1: SAMPLE QUESTIONS

Author:Odisha Board

Odisha Board Biology Solutions for Exercise - Odisha Board Solutions for Exercise 1: SAMPLE QUESTIONS

Attempt the free practice questions from Exercise 1: SAMPLE QUESTIONS with hints and solutions to strengthen your understanding. Bureau's Higher Secondary Biology Class 12 solutions are prepared by Experienced Embibe Experts.

Questions from Odisha Board Solutions for Exercise 1: SAMPLE QUESTIONS with Hints & Solutions

HARD

12th Odisha Board

IMPORTANT

Bureau's Higher Secondary Biology Class 12>Chapter 12 - Principles and Process of Biotechnology>SAMPLE QUESTIONS>Q 2

Detection of desired DNA fragment by the emission of ionizing radiation is known as _____.

HARD

12th Odisha Board

IMPORTANT

Bureau's Higher Secondary Biology Class 12>Chapter 12 - Principles and Process of Biotechnology>SAMPLE QUESTIONS>Q 2

The conjoint structure formed by the joining of the vector DNA and the target DNA fragment is known as _____.

MEDIUM

12th Odisha Board

IMPORTANT

Bureau's Higher Secondary Biology Class 12>Chapter 12 - Principles and Process of Biotechnology>SAMPLE QUESTIONS>Q 3

Answer in one word: Transfer of a DNA fragment into a host cell in a medium by passing brief pulses of electric current through the medium.

EASY

12th Odisha Board

IMPORTANT

Bureau's Higher Secondary Biology Class 12>Chapter 12 - Principles and Process of Biotechnology>SAMPLE QUESTIONS>Q 3

Answer in one word: The instrument used in PCR amplification.

HARD

12th Odisha Board

IMPORTANT

Bureau's Higher Secondary Biology Class 12>Chapter 12 - Principles and Process of Biotechnology>SAMPLE QUESTIONS>Q 4

Match the words of Group A with those of group B to make meaningful pairs:

Group A	Group B
Polyacrylamide	Agrobacterium tumefaciens
Southern blotting	Thermocycler
Plasmid	Protein electrophoresis
Agarose gel	Denaturation
Cos	Taq polymerase
Tumor inducing plasmid	Cloning vehicle
Recombinant DNA	Molecular marker
DNA coated tungsten particles	Nucleic acid electrophoresis
Insertional inactivation	Chimeric DNA
Breaking of interchain hydrogen bonds	Particle gun
Equipment for PCR amplification	Screening of clones
Ampicillin resistance gene	Blotting of DNA
Thermophilus aquaticus	Cohesive site

EASY

12th Odisha Board

IMPORTANT

Bureau's Higher Secondary Biology Class 12>Chapter 12 - Principles and Process of Biotechnology>SAMPLE QUESTIONS>Q 1

Define biotechnology.

EASY

12th Odisha Board

IMPORTANT

Bureau's Higher Secondary Biology Class 12>Chapter 12 - Principles and Process of Biotechnology>SAMPLE QUESTIONS>Q 1

What is polymerase chain reaction (PCR)?

HARD

12th Odisha Board

IMPORTANT

Bureau's Higher Secondary Biology Class 12>Chapter 12 - Principles and Process of Biotechnology>SAMPLE QUESTIONS>Q 1

Describe briefly about the making of a recombinant DNA.

Odisha Board Solutions for Exercise 1: SAMPLE QUESTIONS

Odisha Board Biology Solutions for Exercise - Odisha Board Solutions for Exercise 1: SAMPLE QUESTIONS

Questions from Odisha Board Solutions for Exercise 1: SAMPLE QUESTIONS with Hints & Solutions

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