HARD
AS and A Level
IMPORTANT
Earn 100
A gene construct is one or more genes together with regulatory base sequences which are inserted into a vector to produce recombinant DNA. Gene constructs often contain a marker gene, such as enhanced green fluorescent protein ( GFP). Explain why marker genes, such as enhanced GFP, are included in gene constructs.
Important Questions on Genetic Technology
MEDIUM
AS and A Level
IMPORTANT
A gene construct is one or more genes together with regulatory base sequences which are inserted into a vector to produce recombinant DNA. Gene constructs often contain a marker gene, such as enhanced green fluorescent protein (GFP). Explain the advantage of using an enzyme as a marker gene.
HARD
AS and A Level
IMPORTANT
AqauAdvantage® salmon are genetically modified female Atlantic salmon, Salmo salar. These fish have a single copy of opAFP-GHc2, which is a gene construct prepared from a structural gene from chinook salmon, Oncorhynchus tshawytscha, that codes for a growth hormone and a promoter from the ocean pout, Zoarces americanus. Outline one method in which the gene that codes for growth hormone may have been obtained from O. tshawytscha.
HARD
AS and A Level
IMPORTANT
AqauAdvantage® salmon are genetically modified female Atlantic salmon, Salmo salar. These fish have a single copy of opAFP-GHc2, which is a gene construct prepared from a structural gene from chinook salmon, Oncorhynchus tshawytscha, that codes for a growth hormone and a promoter from the ocean pout, Zoarces americanus. Explain why it is necessary to include a promoter in the construct.
HARD
AS and A Level
IMPORTANT
AqauAdvantage® salmon are genetically modified female Atlantic salmon, Salmo salar. These fish have a single copy of opAFP-GHc2, which is a gene construct prepared from a structural gene from chinook salmon, Oncorhynchus tshawytscha, that codes for a growth hormone and a promoter from the ocean pout, Zoarces americanus. Suggest how the components of the gene construct were assembled.
HARD
AS and A Level
IMPORTANT
AqauAdvantage® salmon are genetically modified female Atlantic salmon, Salmo salar. These fish have a single copy of opAFP-GHc2, which is a gene construct prepared from a structural gene from chinook salmon, Oncorhynchus tshawytscha, that codes for a growth hormone and a promoter from the ocean pout, Zoarces americanus. Outline how the gene construct is inserted into cells of S. salar.
HARD
AS and A Level
IMPORTANT
The GM salmon are all triploid (3n). Suggest the advantages of making the salmon triploid.
HARD
AS and A Level
IMPORTANT
These GM salmon are grown in facilities in Panama, but for many years they were not approved for human consumption by the Food and Drug Administration in the USA. Suggest why it has proved difficult for GM salmon to receive approval for human consumption.
HARD
AS and A Level
IMPORTANT
Explain how it is possible to predict the primary structure of a protein from a gene sequence.
