MEDIUM
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A polymerase chain reaction (PCR) for amplification of DNA fragments involves multiple cycles of thermal denaturation, annealing and extension steps in each cycle. Because of the heat denaturation step, a thermostable polymerase is required for performing PCR. An experimenter had all the resources except the thermostable DNA polymerase to perform a PCR. The experimenter can use _____ to substitute for thermostable DNA polymerase.
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Important Questions on Biotechnology - Principles and Processes
EASY
Rapid antigen test and RT-PCR are the two diagnostic tests for Covid virus. PCR is a molecular diagnostic tool, that stands for:
MEDIUM
Which one among the following primer pairs would amplify the fragment of given below?
MEDIUM
Match the following techniques or instruments with their usage:
| (a) | Bioreactor | (i) | Separation of DNA fragments |
| (b) | Electrophoresis | (ii) | Production of large quantities of products |
| (c) | (iii) | Detection of pathogen, based on antigen-antibody reaction | |
| (d) | (iv) | Amplification of nucleic acids |
Select the correct option from following:
HARD
Which of the following is not a component of downstream processing?
EASY
The Taq polymerase enzyme is obtained from:
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Taq polymerase that finds its application in PCR is obtained from:
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To obtain reliable results from PCR machine, the process can be carried out for about ______ cycle.
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Which ONE of the following is NOT essential for Polymerase Chain Reaction (PCR)?
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Nowadays, the early diagnosis of bacterial or viral infection in humans is possible using
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From the given combinations of steps in PCR, identify the enzyme dependent step/s.
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During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
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The process of separation and purification of expressed protein before marketing is called
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Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?
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Stirred-tank bioreactors have been designed for:
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Which of the following is not an application of PCR (Polymerase Chain Reaction)?
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Arrange the following steps of PCR in a correct order ?
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If the initial number of templets DNA molecules in a PCR reaction is 1000, the number of product DNA molecules at the end of 20 cycles will be closest to
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Suppose DNA samples collected for DNA fingerprinting analysis are less than the required quantity. Which of the following techniques is helpful to make the samples sufficient for above analysis?
MEDIUM
Select the correct steps involved in amplification of gene
HARD
The correct order of steps in Polymerase Chain Reaction (PCR) is
