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Absence of -galactosidase activity in recombinant is
(a)Observed in blue colonies with intact gene
(b)Observed in blue colonies with disrupted gene
(c)Observed in white colonies with intact gene
(d)Observed white colonies with inactivated gene

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Important Questions on Biotechnology - Principles and Processes
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PCR technique using Taq-Polymerase enables directly the ------a------ of billions of copies of an original piece of ------b----- in a tube within minutes. Select the option which fills the blanks and ) correctly?

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The figure below is the diagrammatic representation of a typical agarose gel electrophoresis.
Choose the correct option.

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Amplification of gene of interest by using PCR for cycles may go upto

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The thermostable enzymes, 'Taq’ and 'Pfu', isolated from thermophilic bacteria are:

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Read statements 'A' and 'B' and choose the correct option.
Statement A : Agrobacterium tumefaciens causes crown gall in dicots
Statement B : Agrobacterium tumefaciens enters the host through wound and injuries in certain plants.

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The correct order of steps in Polymerase Chain Reaction (PCR) is:

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While constructing the first recombinant DNA molecule, Stanley Cohen and Herbert Boyer isolated plasmid from A while B acted as host for the recombinant plasmid.
Select the option which gives correct answer for blanks in above statements.

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Select the option which gives correct name for the restriction enzyme isolated from Haemophilus influenzae.
