HARD
Earn 100
As opposed to DNA replication within the cell, discontinuous synthesis of DNA does NOT occur in a polymerase chain reaction (PCR). Why?
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Important Questions on Principles and Processes of Biotechnology
HARD
Name the most commonly used bioreactor in biotechnology labs. Mention the most essential components this bioreactor must have so as to provide the optimum conditions to the culture medium, resulting in production of large volume of the desired product.
MEDIUM
Features of sparged stirred tank bioreactor that differ from simple stirred bioreactors
I) More bubble formation
II) Decrease the surface area for transfer
III) It has foam control system
EASY
How has the development of bioreactor helped in biotechnology ?
HARD
Which of the following is not a component of downstream processing?
MEDIUM
Which one among the following primer pairs would amplify the fragment of given below?
HARD
What does PCR stand for? Describe the different steps of PCR.
EASY
Name the most commonly used bioreactor and describe its working.
MEDIUM
Describe briefly about bioreactors.
EASY
Which ONE of the following is NOT essential for Polymerase Chain Reaction (PCR)?
MEDIUM
Match the following techniques or instruments with their usage:
| (a) | Bioreactor | (i) | Separation of DNA fragments |
| (b) | Electrophoresis | (ii) | Production of large quantities of products |
| (c) | (iii) | Detection of pathogen, based on antigen-antibody reaction | |
| (d) | (iv) | Amplification of nucleic acids |
Select the correct option from following:
EASY
Stirred-tank bioreactors have been designed for:
MEDIUM
Select the correct steps involved in amplification of gene
MEDIUM
What is a bioreactor? Explain important features of a stirred tank bioreactor.
MEDIUM
If the initial number of templets DNA molecules in a PCR reaction is 1000, the number of product DNA molecules at the end of 20 cycles will be closest to
HARD
Explain the various steps of polymerase chain reaction with the help of diagram.
MEDIUM
What is the full form of PCR? State its one use in biotechnology.
HARD
Explain briefly about PCR.
EASY
Arrange the following steps of PCR in a correct order ?
EASY
Nowadays, the early diagnosis of bacterial or viral infection in humans is possible using
EASY
The process of separation and purification of expressed protein before marketing is called
