Blue-white screening is used as a method for screening of recombinant strain.

Identify the labeled parts $1, 2, 3, 4$ in the given figure?

Assertion ($\mathrm{A}$): All Eukaryotic cells have $\mathrm{RNAi}$ for cellular defence.

Reason ($\mathrm{R}$): $\mathrm{RNAi}$ silences $\mathrm{mRNA}$ and prevents translation.

Assertion (A): The fragments of DNA generated by the action of restriction endonucleases are separated by polyacrylamide gel electrophoresis.

Reason (R)： The separated DNA fragments are visualised as bright orange coloured bands after staining with Ethidium Bromide followed by exposure to $\mathrm{UV}$ - radiation.

Which of the following is true?

Study the following statement regarding rDNA technology and select the incorrect ones ?
(i) Taq polymerase extends the primers using the nucleotides provided in the reaction
(ii) Antibiotic resistance genes are considered as desirable genes in rDNA technology
(iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis
(iv) Transformation is a procedure through which a piece of DNA is introduced in a host bacterium
(v) To produce higher yields of a desired protein, host cells can be multiplied in a continuous culture
(vi) Downstream processing is one of the steps of PCR

Identify the labels $\mathrm{M}$ and $\mathrm{N}$ in the following Agarose gel electrophoresis representation.