Match the items given in Column I with their uses in Column II and choose the correct option. Column I Column II A. Escherichia coli i) Thermostable DNA polymerase B. Thermus aquaticus ii) Restriction endonuclease C. Agrobacterium tumefaciens iii) Gene gun D. Biolistic iv) Ti plasmid

A - ii), B - i), C - iv), D - iii)

A - i), B - ii), C - iv), D - iii)

A - iv), B - i), C - iii), D - ii)

A - iii), B - iv), C - ii), D - i)

The construction of first artificial recombinant DNA molecule was by:

Stanley Cohen and Herbert Boyer using the plasmid of Salmonella typhimurium

Stanley Cohen and Herbert Boyer using the plasmid of Escherichia coli

Arber, Smith and Nathans using the plasmid of Salmonella typhimurium

Arber, Smith and Nathans using the plasmid of Agrobacterium tumefaciens

Restriction endonuclease breaks bond between the:

Sugar-phosphate backbone of nucleic acid

N-glycosidic bond between sugar and base

Biolistics (gene-gun) is suitable for

Constructing recombinant DNA by joining with vectors

Which one of the following represents a palindromic sequence in DNA?

5' - GAATTC - 3' 3' - CTTAAG - 5'

5' - CCAATG - 3' 3' - GAATCC - 5'

5' - CATTAG - 3' 3' - GATAAC - 5'

5' - GATACC - 3' 3' - CCTAAG - 5'

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Important Questions on Biotechnology - Principles and Processes

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 56

Which of the following is an application of PCR?

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 57

One of the key factors which makes the plasmid the vector in genetic engineering is

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 3>Q 1

Choose the wrong statement from the following:

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 3>Q 2

Read the given statements and select the correct option.
Statement 1: The tumour inducing plasmid (Ti plasmid) acts as a cloning vector in recombinant DNA technology.
Statement 2: The Ti plasmid which is used in the mechanisms of delivering genes to a cell remains pathogenic.

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 3>Q 3

Ideal cloning vector should have:

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 3>Q 4

Read the given statements and select the correct option.
Statement 1: The cloning vector is required to have very few, preferably single, recognition sites for the commonly used restriction enzymes.
Statement 2: Presence of more than one recognition sites within a cloning vector will generate several fragments, which will complicate the process of gene cloning.

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 3>Q 5

Match the terms given in Column I with their definitions in Column II and select the correct answer from codes given below.

	Column I		Column II
$(a)$	Transformation	$(i)$	Sequences cut by restriction enzymes
$(b)$	Recognition	$(i i)$	Process by which DNA fragments are separated based on their size
$(c)$	Gel electrophoresis	$(i i i)$	Plasmid DNA that has incorporated human DNA
$(d)$	Recombinant DNA	$(i v)$	Process by which bacteria take up pieces of DNA from the environment

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 3>Q 6

Match the items given in Column I with their uses in Column II and choose the correct option.

Column I	Column II
A. Escherichia coli	i) Thermostable DNA polymerase
B. Thermus aquaticus	ii) Restriction endonuclease
C. Agrobacterium tumefaciens	iii) Gene gun
D. Biolistic	iv) Ti plasmid

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Important Questions on Biotechnology - Principles and Processes

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