MEDIUM
Earn 100
Choose the correct statements.
A) If any protein encoding gene is expressed in a heterologous host then it is called recombinant protein.
B) With the presence of insertional inactivation of the -galactosidase, the colonies which do not produce blue colour are called recombinant colonies.
C) The origin of replication sequence act as closing site.
D) Palindromic nucleotide sequence of DNA is identified by exonucleases.
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Important Questions on Biotechnology and its Application
HARD
What is recombinant DNA technology? How is recombinant DNA prepared by using restriction enzymes? Describe briefly with the help of diagram.
HARD
List four applications of genetically modified plants.
EASY
Explain Agrobacterium mediated gene transfer.
HARD
By which two properties active and facilitated transport differs in different transport processes?
EASY
The construction of first artificial recombinant DNA molecule was by:
MEDIUM
What is genetic engineering?
MEDIUM
What is genetic engineering? How is it beneficial?
EASY
Which DNA fragment of a vector that helps in identifying and eliminating non-transformant cells and permit the growth of transformant cells?
EASY
A segment of DNA molecule comprises of 546 nucleotides. How many cytosine nucleotides would be present in it if the number of adenine nucleotides is 96 ?
EASY
Write short note on the following:
Gene cloning
EASY
Give the full form of EFB, Whether it is European Federation of Biotechnology or European Federation of Biology.
EASY
H. Boyer S. Cohen are famous for their research work on
EASY
Who discovered the transfer of genetic material from one bacterium to another bacterium through pilus?
MEDIUM
Describe gene cloning.
MEDIUM
In a restriction enzyme digestion experiment, a fragment of kilobases length is used. This fragment harbors three restriction sites. After the restriction digestion of this fragment with EcoRI, fragments corresponding to base pairs are observed in the agarose gel electrophoresis . Predict the positions of the restriction site in the undigested -fragment which will result in the observed bands in .
EASY
Which of the following is a restriction endonuclease?
MEDIUM
Assertion (A): Chromogenic substrates give blue-coloured colonies if the plasmid does not have an insert.
Reason (R): Recombinant colonies produce colour in presence of the chromogenic substrates due to the presence of galactosidase.
The correct option among the following is:
HARD
What is DNA library?
MEDIUM
The core techniques enabled modern biotechnology
Change the phenotype of host-organism by genetic engineering
Chemical engineering process of vaccine production by tissue culture
Manufacture of specific products from eukaryotic cells in bioreactors
Altering the chemistry of genetic material by tissue culture
MEDIUM
Match the organism with its use in biotechnology.
| (a) | Bacillus thuringiensis | (i) | Cloning vector |
| (b) | Thermus aquaticus | (ii) | Construction of first rDNA molecule |
| (c) | Agrobacterium tumefaciens | (iii) | DNA polymerase |
| (d) | Salmonella typhimurium | (iv) | Cry proteins |
