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Cloning vectors are called vehicle DNA.

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Important Questions on Genetics of the Future

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The construction of the first recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of
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Identify the correct statements.
(A) In gel electrophoresis, DNA fragments resolve according to their size.
(B) It is not necessary to digest the bacterial cells with lysozyme to isolate DNA
(C) In PCR, DNA polymerase extends the primers using the nucleotides.
(D) During the isolation of DNA, ribonuclease is added along with protease
(E) Agarose gel electrophoresis is employed to check the progression of restriction enzyme digestion.
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Name any two natural cloning vectors. Give reasons that make them act as cloning vectors. Write the two characteristics the engineered vectors are made to possess.
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What is plasmid?
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Science>Biology>Genetics of the Future>Genetic Engineering
The ratio of genes for protein involved in plasmid replication rop codes for proteins, Ori site, restriction sites, antibiotic resistance genes present in E. coli cloning vector pBR322 is
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Give one significant contribution of the following scientist:

H. Boyer

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Explain the roles of the following with the help of an example in recombinant DNA technology

Plasmids

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Name "any one' therapeutic product of blood proteins made by using r-DNA technology.
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Science>Biology>Genetics of the Future>Genetic Engineering
Rop-gene which codes the proteins involved in the replication of the plasmid pBR322 in E. coli is located at the restriction site of:
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Science>Biology>Genetics of the Future>Genetic Engineering

A gene is inserted into the PvuII site of the cloning vector (given below) and transformed into E. coli cells. Which one of the following statements is then true?

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What is called gene cloning?
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Science>Biology>Genetics of the Future>Genetic Engineering
Which vector can clone only a small fragment of DNA?
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Science>Biology>Genetics of the Future>Genetic Engineering
Write differences between plasmid DNA and chromosomal DNA.
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Science>Biology>Genetics of the Future>Genetic Engineering
Explain the various steps involved in the DNA Recombinant technology.
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Science>Biology>Genetics of the Future>Genetic Engineering
What is a plasmid? Write briefly on the main aims of biotechnology.
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Science>Biology>Genetics of the Future>Genetic Engineering
What will happen when a foreign DNA is introduced at Sal I place in E.coli plasmid cloning vector? Choose the correct option:
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Science>Biology>Genetics of the Future>Genetic Engineering
Enlist the steps involved in rDNA technology. 
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The given figure is the diagrammatic representation of the E. Coli vector pBR 322. Which one of the given options correctly identifies its certain components?

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Match the organism with its use in biotechnology.

(a) Bacillus thuringiensis (i) Cloning vector
(b) Thermus aquaticus (ii) Construction of first rDNA molecule
(c) Agrobacterium tumefaciens (iii) DNA polymerase
(d) Salmonella typhimurium (iv) Cry proteins
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Examine the diagram and identify the correction option.

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