DNA cloning process can be characterised as a complex process. It plays an essential role in the genetic engineering. The total DNA from a cell is isolated and converted into random fragments of relatively smaller size for sequencing. Then it is cloned in a suitable host using specialized vectors. The cloning results in the amplification of DNA fragments which are used for sequencing the bases. Yeast is used as the host in the vector YAC. YAC stands for yeast artificial chromosome.

Match the organism with its use in biotechnology. (a) Bacillus thuringiensis (i) Cloning vector (b) Thermus aquaticus (ii) Construction of first rDNA molecule (c) Agrobacterium tumefaciens (iii) DNA polymerase (d) Salmonella typhimurium (iv) Cry proteins

(a)-(iv), (b)-(iii), (c)-(i), (d)-(ii)

(a)-(iii), (b)-(ii), (c)-(iv), (d)-(i)

(a)-(iii), (b)-(iv), (c)-(i), (d)-(ii)

(a)-(ii), (b)-(iv), (c)-(iii), (d)-(i)

MEDIUM

Earn 100

Define YAC.

Important Questions on Principles and Processes of Biotechnology

MEDIUM

Biology>Biotechnology>Principles and Processes of Biotechnology>Tools for Genetic Engineering

Statement (S): Less than $2 %$ of the human genome encodes for functional units called 'proteins'.

Reason (R): The majority of the human genome is regulatory in nature.

Which of the following is true?

EASY

Biology>Biotechnology>Principles and Processes of Biotechnology>Tools for Genetic Engineering

What is the importance of E.Coli in biotechnology ?

MEDIUM

Biology>Biotechnology>Principles and Processes of Biotechnology>Tools for Genetic Engineering

Expressed sequence tags (ESTs) refers to:

EASY

Biology>Biotechnology>Principles and Processes of Biotechnology>Tools for Genetic Engineering

Which vector can clone only a small fragment of DNA?

EASY

Biology>Biotechnology>Principles and Processes of Biotechnology>Tools for Genetic Engineering

Which of the following is a restriction endonuclease?

EASY

Biology>Biotechnology>Principles and Processes of Biotechnology>Tools for Genetic Engineering

DNA's precipitation out of a mixture of biomolecules can be achieved by treatment with:

HARD

Biology>Biotechnology>Principles and Processes of Biotechnology>Tools for Genetic Engineering

Write an essay on DNA recombinant technology.

HARD

Biology>Biotechnology>Principles and Processes of Biotechnology>Tools for Genetic Engineering

In biotechnology, which enzymes are called ‘molecular scissors’ and ‘molecular glue’?

EASY

Biology>Biotechnology>Principles and Processes of Biotechnology>Tools for Genetic Engineering

Commonly used vectors for human genome sequencing are:

EASY

Biology>Biotechnology>Principles and Processes of Biotechnology>Tools for Genetic Engineering

Name the enzymes involved in making hybrid DNA.

MEDIUM

Biology>Biotechnology>Principles and Processes of Biotechnology>Tools for Genetic Engineering

Match the organism with its use in biotechnology.

(a)	Bacillus thuringiensis	(i)	Cloning vector
(b)	Thermus aquaticus	(ii)	Construction of first rDNA molecule
(c)	Agrobacterium tumefaciens	(iii)	DNA polymerase
(d)	Salmonella typhimurium	(iv)	Cry proteins

EASY

Biology>Biotechnology>Principles and Processes of Biotechnology>Tools for Genetic Engineering

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

EASY

Biology>Biotechnology>Principles and Processes of Biotechnology>Tools for Genetic Engineering

Ti

plasmid is obtained from ___.

MEDIUM

Biology>Biotechnology>Principles and Processes of Biotechnology>Tools for Genetic Engineering

Study the following statements
I) Gene that codes for the synthesis of dystrophin is the largest human gene
II) Chromosome $21$ contains the highest number of genes
III Endonucleases are used to cut the DNA at specific region
IV) Whole hereditary information encoded in DNA of an organism is called genome

Among the above identify the correct statements

EASY

Biology>Biotechnology>Principles and Processes of Biotechnology>Tools for Genetic Engineering

The ratio of genes for protein involved in plasmid replication rop codes for proteins, Ori site, restriction sites, antibiotic resistance genes present in E. coli cloning vector