EASY
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Denaturation is a step in the procedure called Polymerase Chain Reaction. This step does not involve which of the following process?
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Important Questions on Biotechnology - Principles and Processes
MEDIUM
Match the following techniques or instruments with their usage:
| (a) | Bioreactor | (i) | Separation of DNA fragments |
| (b) | Electrophoresis | (ii) | Production of large quantities of products |
| (c) | (iii) | Detection of pathogen, based on antigen-antibody reaction | |
| (d) | (iv) | Amplification of nucleic acids |
Select the correct option from following:
EASY
Suppose DNA samples collected for DNA fingerprinting analysis are less than the required quantity. Which of the following techniques is helpful to make the samples sufficient for above analysis?
EASY
To obtain reliable results from PCR machine, the process can be carried out for about ______ cycle.
EASY
During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
EASY
Stirred-tank bioreactors have been designed for:
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Taq polymerase that finds its application in PCR is obtained from:
MEDIUM
Which one among the following primer pairs would amplify the fragment of given below?
EASY
Which ONE of the following is NOT essential for Polymerase Chain Reaction (PCR)?
HARD
Which of the following is not a component of downstream processing?
EASY
Nowadays, the early diagnosis of bacterial or viral infection in humans is possible using
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Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?
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The process of separation and purification of expressed protein before marketing is called
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Which of the following is not an application of PCR (Polymerase Chain Reaction)?
EASY
Rapid antigen test and RT-PCR are the two diagnostic tests for Covid virus. PCR is a molecular diagnostic tool, that stands for:
HARD
The correct order of steps in Polymerase Chain Reaction (PCR) is
EASY
Arrange the following steps of PCR in a correct order ?
MEDIUM
If the initial number of templets DNA molecules in a PCR reaction is 1000, the number of product DNA molecules at the end of 20 cycles will be closest to
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From the given combinations of steps in PCR, identify the enzyme dependent step/s.
MEDIUM
Select the correct steps involved in amplification of gene
EASY
The Taq polymerase enzyme is obtained from:
