Distinguish between exonuclease and endonuclease.

Important Questions on Biotechnology: Principles and Processes

MEDIUM

12th Andhra Pradesh Board

IMPORTANT

Intermediate Second Year Botany>Chapter 11 - Biotechnology: Principles and Processes>Questions>Q 7

What does 'H' 'in' 'd' and 'III' refer to in Hind III ?

MEDIUM

12th Andhra Pradesh Board

IMPORTANT

Intermediate Second Year Botany>Chapter 11 - Biotechnology: Principles and Processes>Questions>Q 8

Restriction enzymes should not have more than one site of action in cloning site of a vector. Comment.

MEDIUM

12th Andhra Pradesh Board

IMPORTANT

Intermediate Second Year Botany>Chapter 11 - Biotechnology: Principles and Processes>Questions>Q 9

What does 'competent' refer to in 'competent cells' used in transformation experiments?

MEDIUM

12th Andhra Pradesh Board

IMPORTANT

Intermediate Second Year Botany>Chapter 11 - Biotechnology: Principles and Processes>Questions>Q 10

What is the significance of adding proteases at the time of isolation of genetic material (DNA).

MEDIUM

12th Andhra Pradesh Board

IMPORTANT

Intermediate Second Year Botany>Chapter 11 - Biotechnology: Principles and Processes>Questions>Q 11

While doing a 'PCR' 'denaturation step is missed. What will be its effect on the process?

MEDIUM

12th Andhra Pradesh Board

IMPORTANT

Intermediate Second Year Botany>Chapter 11 - Biotechnology: Principles and Processes>Questions>Q 12

What modification is done on the 'Ti' plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?

MEDIUM

12th Andhra Pradesh Board

IMPORTANT

Intermediate Second Year Botany>Chapter 11 - Biotechnology: Principles and Processes>Questions>Q 13

What is meant by gene cloning?

HARD

12th Andhra Pradesh Board

IMPORTANT

Intermediate Second Year Botany>Chapter 11 - Biotechnology: Principles and Processes>Questions>Q 14

Decide the ratio between the ester bonds and hydrogen bonds that are broken in each palindromic sequence of a DNA when treated with EcoRI during the formation of sticky ends?