Write the methods to introduce alien DNA into Host cells.

DNA can be introduced into host cells through some methods. These methods are given below: Microinjection : The DNA is incorporated through a glass needle in the nuclei of the cells. Gene gun : In this process, the metal particles are loaded with DNA, and are bombarded into the cells with high velocity. Electroporation : The cells are exposed to a high electric field, which leads to the high permeability of cells and the DNA is introduced to cells.

Write the restriction site of EcoRI enzyme.

EcoRI can be characterised as a restriction enzyme isolated from the E.coli bacteria in which the R represents the particular strain named RY13 and I represent that it was the first enzyme isolated from this strain. This enzyme is responsible for the cleavage of DNA double helix into fragments at some specific sites. The restriction site of the EcoRI enzyme is 5'-GAATTC-3' and the complementary strand will be 3'-CTTAAG-5'.