Biolistics (gene-gun) is suitable for

Constructing recombinant DNA by joining with vectors

Identify the palindromic sequence.

5 ' - GAATTC - 3 ' 3 ' - CTTAAG - 5 '

5 ' - CATTC - 3 ' 3 ' - GTAAG - 5 '

5 ' - AACTC - 3 ' 3 ' - TTGAG - 5 '

5 ' - CTTCC - 3 ' 3 ' - GAAGG - 5 '

Which one of the following represents a palindromic sequence in DNA?

5' - GAATTC - 3' 3' - CTTAAG - 5'

5' - CCAATG - 3' 3' - GAATCC - 5'

5' - CATTAG - 3' 3' - GATAAC - 5'

5' - GATACC - 3' 3' - CCTAAG - 5'

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Alpha Question Bank for Medical: Biology>Chapter 33 - Biotechnology - Principles and Processes>Exercise-1>Q 2

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Each restriction endonuclease recognises a .......... sequences in the DNA.

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Important Questions on Biotechnology - Principles and Processes

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Alpha Question Bank for Medical: Biology>Chapter 33 - Biotechnology - Principles and Processes>Exercise-1>Q 3

Which type of restriction enzymes are used in recombinant DNA technology?

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Alpha Question Bank for Medical: Biology>Chapter 33 - Biotechnology - Principles and Processes>Exercise-1>Q 4

Restriction endonucleases are enzymes which

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Alpha Question Bank for Medical: Biology>Chapter 33 - Biotechnology - Principles and Processes>Exercise-1>Q 5

Polymerase chain reaction involves use of a DNA polymerase enzyme which is

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Alpha Question Bank for Medical: Biology>Chapter 33 - Biotechnology - Principles and Processes>Exercise-1>Q 6

Transfer of DNA bands from an agarose gel to a nitrocellulose or nylon membrane is referred to as

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Alpha Question Bank for Medical: Biology>Chapter 33 - Biotechnology - Principles and Processes>Exercise-1>Q 7

During "gene cloning" which is called as "gene taxi"

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Alpha Question Bank for Medical: Biology>Chapter 33 - Biotechnology - Principles and Processes>Exercise-1>Q 8

Restriction endonucleases were used by Cohen and Boyer's DNA experiments for

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Alpha Question Bank for Medical: Biology>Chapter 33 - Biotechnology - Principles and Processes>Exercise-1>Q 9

Identify the correct sequence of performing PCR.
(a) Annealing of primers
(b) Extensions of primers using the nucleotides provided in the reaction and the genomic DNA as template.
(c) Denaturation of the double stranded DNA.
(d) A segment of DNA is amplified approximately a billion times.

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Alpha Question Bank for Medical: Biology>Chapter 33 - Biotechnology - Principles and Processes>Exercise-2>Q 1

Restriction endonucleases are enzymes that cleave DNA molecules into smaller fragments. Which type of bond do they act on?