EASY
NEET
IMPORTANT
Earn 100

EcoRI recognizes the palindrome 5' GAATTC 3'. How many fragments are obtained upon digesting pBR322 with Sal I?

75% studentsanswered this correctly

Important Questions on Biotechnology - Principles and Processes

MEDIUM
NEET
IMPORTANT

Match column I and column II and choose the correct option.

Column I Column II
a. Selectable marker (i) DNA precipitation
b. Sticky ends (ii) EcoRI
c. Chilled ethanol (iii) Identify transformed cells
d. Plasmids (iv) Autonomous replication

 

EASY
NEET
IMPORTANT

Read statements A and B and select the correct option given below.

Statement-A : DNA is a hydrophilic molecule, thus it cannot pass through cell membrane.
Statement-B : Electroporation creates transient microscopic pores in cell membrane making entry of DNA molecules into the cell easier.

EASY
NEET
IMPORTANT
Tumor inducing plasmid delivers a piece of DNA known as 'T-DNA' to transform
EASY
NEET
IMPORTANT
The intercalating agent that stacks between base pairs of DNA and helps in visualizing them under UV light is
EASY
NEET
IMPORTANT
Fragments of DNA formed after treatment with endonucleases are separated by the technique of
EASY
NEET
IMPORTANT
Stirred-tank bioreactors have been designed for
MEDIUM
NEET
IMPORTANT
Absence of β-galactosidase activity in recombinant pUC18 is
EASY
NEET
IMPORTANT
PCR technique using Taq-Polymerase enables directly the ------a------ of billions of copies of an original piece of ------b----- in a tube within minutes. Select the option which fills the blanks a and b ) correctly?