Match column I and column II and choose the correct option. Column I Column II a. Selectable marker (i) DNA precipitation b. Sticky ends (ii) EcoRI c. Chilled ethanol (iii) Identify transformed cells d. Plasmids (iv) Autonomous replication

a ( iii ) , b ( ii ) , c ( i ) , d ( iv )

a ( ii ) , b ( iii ) , c ( i ) d ( iv )

a ( iii ) , b ( ii ) , c ( iv ) , d ( i )

a ( iii ) , b ( i ) , c ( ii ) , d ( iv )

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Gamma Question Bank for Medical Biology>Chapter 33 - Biotechnology - Principles and Processes>Assignment>Q 4

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Earn 100

$EcoRI$ recognizes the palindrome $5^{'}$ GAATTC $3^{'}$ . How many fragments are obtained upon digesting $pBR 322$ with $Sal I$ ?

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Important Questions on Biotechnology - Principles and Processes

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Gamma Question Bank for Medical Biology>Chapter 33 - Biotechnology - Principles and Processes>Assignment>Q 5

Match column I and column II and choose the correct option.

Column I	Column II
a. Selectable marker	(i) DNA precipitation
b. Sticky ends	(ii) EcoRI
c. Chilled ethanol	(iii) Identify transformed cells
d. Plasmids	(iv) Autonomous replication

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Gamma Question Bank for Medical Biology>Chapter 33 - Biotechnology - Principles and Processes>Assignment>Q 6

Read statements A and B and select the correct option given below.

Statement-A : DNA is a hydrophilic molecule, thus it cannot pass through cell membrane.
Statement-B : Electroporation creates transient microscopic pores in cell membrane making entry of DNA molecules into the cell easier.

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Gamma Question Bank for Medical Biology>Chapter 33 - Biotechnology - Principles and Processes>Assignment>Q 7

Tumor inducing plasmid delivers a piece of DNA known as 'T-DNA' to transform

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Gamma Question Bank for Medical Biology>Chapter 33 - Biotechnology - Principles and Processes>Assignment>Q 8

The intercalating agent that stacks between base pairs of DNA and helps in visualizing them under UV light is

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Gamma Question Bank for Medical Biology>Chapter 33 - Biotechnology - Principles and Processes>Assignment>Q 9

Fragments of DNA formed after treatment with endonucleases are separated by the technique of

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Gamma Question Bank for Medical Biology>Chapter 33 - Biotechnology - Principles and Processes>Assignment>Q 10

Stirred-tank bioreactors have been designed for

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Gamma Question Bank for Medical Biology>Chapter 33 - Biotechnology - Principles and Processes>Assignment>Q 11

Absence of

β

-galactosidase activity in recombinant

pUC 18

is

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Gamma Question Bank for Medical Biology>Chapter 33 - Biotechnology - Principles and Processes>Assignment>Q 12

PCR technique using Taq-Polymerase enables directly the ------a------ of billions of copies of an original piece of ------b----- in a tube within minutes. Select the option which fills the blanks