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Electroporation is used as a physical method of gene transfer,

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Important Questions on Biotechnology and Bioinformatics

MEDIUM
Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
Which among the following is a selectable marker in pBR – 322 ?
HARD
Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
Give an account of the Blue-White Method of selection of recombinants.
HARD
Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
By particles of which metal recombinant DNA is coated in particle bombardment?
MEDIUM
Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
Explain any two methods for introduction of recombinant DNA. into host cell.
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109 bacteria were spread on an agar plate containing penicillin. After incubation overnight at 37oC 10 bacterial colonies were observed on the plate. That the colonies are likely to be resistant to penicillin can be tested by
EASY
Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
Write the methods to introduce alien DNA into Host cells.
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Write short note on the following :

Gene gun

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Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
The crops engineered with Bt-toxin are resistant/tolerant to:
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Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
In biotechnology applications, a selectable marker is incorporated in a plasmid
HARD
Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
Describe two vector less methods of gene transfer used in rDNA technology.
MEDIUM
Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
Choose the wrong statement from the following:
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Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
A gene whose expression helps to identify transformed cell is known as
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Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
An antibiotic resistance gene in a vector usually helps in the selection of
MEDIUM
Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
What is gene-gun?
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Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
Which part of the tobacco plant is infected by Meloidogyne incognita?
EASY
Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
Biolistics method is suitable for gene transfer into ___.
EASY
Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
Which of the following is the first step for genetically modifying an organism?
MEDIUM
Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture

Assertion (A): Chromogenic substrates give blue-coloured colonies if the plasmid does not have an insert.

Reason (R): Recombinant colonies produce colour in presence of the chromogenic substrates due to the presence of β galactosidase.
The correct option among the following is:

EASY
Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
In cloning vectors, antibiotic resistance genes are helpful for.
EASY
Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
The technique of bombarding plant cells with high velocity microparticle's of gold or tungsten, coated with DNA, is