Describe the experiment carries out by Hershey and Chase. Write the conclusion they arrived at.

Hershey and Chase Experiment to identify the genetic material: Hershey and Chase performed their experiments with bacterium E.coli and bacteriophage T 2 . In this experiment, Hershey and Chase cultured viruses on a medium containing radioactive phosphorus (P 32 ), and some on radioactive sulphur (S 35 ). The radioactive DNA is carried by viruses grown on radioactive phosphorus, the radioactive protein is carried by viruses grown in radioactive sulphur. Radioactive phages were allowed to attach to E.coli bacteria. The virus coats were separated from bacteria by agitating in a blender, the virus particles were isolated from the bacteria on centrifugation. Bacteria infected with viruses containing radioactive DNA were radioactive, whereas bacterial cells infected with virus-containing radioactive protein did not show radioactivity. Hence, they arrived at a conclusion that the genetic material that DNA is passed from the virus to bacteria.

Write a possible RNA strand that can be transcribed from the above DNA molecule formed.

mRNA segments are transcribed in the 5' to 3' direction complementary to the template strand of DNA. The following strand of mRNA will be formed during the process of transcription. DNA :- 3' - A T G C A G T A C G T C G T A - 5' RNA :- 5' - U A C G U C A U G C A G C A U - 3'

Given below is one of the strands of a DNA segment. 3' TACGTACGTACGTACG 5' Write a possible RNA strand that can be transcribed from the above DNA molecule formed.

In the question mentioned above, a short DNA segment with polarity is given, mentioning the nitrogenous bases. According to Chargaff's rule, also known as the complementary base pairing rule, a purine always pairs with the pyrimidine and vice versa. RNA stands for ribonucleic acid is synthesised from the DNA through a process called transcription. The RNA strand produced through transcription contains nitrogenous bases that justify the complementary base pairing rule, but one exception is that uracil pairs with adenine instead of thymine. Thus, the possible RNA strand that can be transcribed from the above DNA molecule formed is: 5' AUGCAUGCAUGCAUGC 3'

E M B I B E

EMBIBE CHAPTER WISE PREVIOUS YEAR PAPERS FOR BIOLOGY>Chapter 6 - Molecular Basis of Inheritance>CBSE-2018>Q 3

EASY

12th CBSE

IMPORTANT

Earn 100

Expand VNTR and describe its role in DNA fingerprinting.

Important Questions on Molecular Basis of Inheritance

EASY

12th CBSE

IMPORTANT

EMBIBE CHAPTER WISE PREVIOUS YEAR PAPERS FOR BIOLOGY>Chapter 6 - Molecular Basis of Inheritance>CBSE-2018>Q 4

List any two applications of DNA fingerprinting technique.

HARD

12th CBSE

IMPORTANT

EMBIBE CHAPTER WISE PREVIOUS YEAR PAPERS FOR BIOLOGY>Chapter 6 - Molecular Basis of Inheritance>CBSE-2018>Q 5

State the ‘Central dogma’ as proposed by Francis Crick. Are there any exceptions to it? Support your answer with a reason and an example.

HARD

12th CBSE

IMPORTANT

EMBIBE CHAPTER WISE PREVIOUS YEAR PAPERS FOR BIOLOGY>Chapter 6 - Molecular Basis of Inheritance>CBSE-2018>Q 6

Explain how the biochemical characterisation (nature) of ‘Transforming Principle’ was determined, which was not defined from Griffith’s experiments.

EASY

12th CBSE

IMPORTANT

EMBIBE CHAPTER WISE PREVIOUS YEAR PAPERS FOR BIOLOGY>Chapter 6 - Molecular Basis of Inheritance>CBSE-2019>Q 7

Differentiate between point mutation and frameshift mutation.

HARD

12th CBSE

IMPORTANT

EMBIBE CHAPTER WISE PREVIOUS YEAR PAPERS FOR BIOLOGY>Chapter 6 - Molecular Basis of Inheritance>CBSE-2019>Q 8

"Use of heavy isotope of nitrogen by Meselson and Stahl demonstrated semi-conservative mode of replication of a DNA molecule." Explain how did they arrive at this conclusion.

MEDIUM

12th CBSE

IMPORTANT

EMBIBE CHAPTER WISE PREVIOUS YEAR PAPERS FOR BIOLOGY>Chapter 6 - Molecular Basis of Inheritance>CBSE-2019>Q 9

Explain the mechanism of translation that occurs in the ribosomes in a prokaryote.

HARD

12th CBSE

IMPORTANT

EMBIBE CHAPTER WISE PREVIOUS YEAR PAPERS FOR BIOLOGY>Chapter 6 - Molecular Basis of Inheritance>CBSE-2020>Q 10

Meselson and Stahl carried out centrifugation in CsCl₂ density gradient to separate:

MEDIUM

12th CBSE