
Explain Molecular diagnosis
Important Questions on Biotechnology and its Applications


Explain briefly
Molecular diagnosis

With regard to insulin choose correct options.
(a) C-peptide is not present in mature insulin.
(b) The insulin produced by rDNA technology has C-peptide.
(c) The pro-insulin has C-peptide.
(d) A-peptide and B-peptide of insulin are interconnected by disulphide bridges.


Broad-spectrum antibiotics



Assertion (A): Molecular fariming is large scale production of biochemicals from plants.
Reason (R) : Transgenic plants are bioreactors for commercial production of antibodies.
The correct option among the following is:


Match the following
List - I | List - II | ||
(a) | First clinical gene therapy | (i) | DNA Amplification |
(b) | Transfection | (ii) | Introduction of genetically modified DNA into a eukaryotic cell |
(c) | Synthetic oligodeoxynucleotides | (iii) | Adenosine deaminase |
(d) | PCR | (iv) | Silencing genes |
The correct answer is




Give the role of:
Tissue growth factor-Beta (TGF-) in gene therapy.



Identify the correct sequence of steps that are followed in gene therapy.
A Altered virus is mixed with cells from patient
B. Therapeutic human gene is inserted into the virus (vector)
C. Cells are collected from the patients
D. Cells from the patient become genetically altered
E. Reintroduction of genetically altered cells into patient
F. Suitable virus (vector) is selected and altered
G The genetically altered cells produce the desired proteins or hormones


