EASY
Earn 100

Explain Molecular diagnosis

Important Questions on Biotechnology and its Applications

HARD
A child is born with ADA deficiency. Suggest and explain a procedure for the possible life-long (permanent) cure.
 
EASY
Mention the chemical change that proinsulin undergoes, to be able to act as mature insulin.
MEDIUM

With regard to insulin choose correct options.

(a) C-peptide is not present in mature insulin.

(b) The insulin produced by rDNA technology has C-peptide.

(c) The pro-insulin has C-peptide.

(d) A-peptide and B-peptide of insulin are interconnected by disulphide bridges.

MEDIUM
Define the following term with a suitable example: 
Broad-spectrum antibiotics
HARD
Define biotechnology. Write the applications of biotechnology in medical science.
HARD
Now a days it is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise its complimentary DNA in a clone of cells, followed by its detection using autoradiography because:
MEDIUM

Assertion (A): Molecular fariming is large scale production of biochemicals from plants.

Reason (R) : Transgenic plants are bioreactors for commercial production of antibodies. 

The correct option among the following is:

MEDIUM

Match the following 

   List - I     List - II
(a) First clinical gene therapy (i) DNA Amplification
(b) Transfection (ii) Introduction of genetically modified DNA into a eukaryotic cell
(c) Synthetic oligodeoxynucleotides (iii) Adenosine deaminase
(d) PCR (iv) Silencing genes

The correct answer is

HARD
What is meant by the term ‘broad spectrum antibiotics? Explain with an example.
MEDIUM
A child is born with ADA deficiency. Name any other possible treatment for this disease.
MEDIUM

Give the role of:

Tissue growth factor-Beta (TGF-β) in gene therapy.

MEDIUM
Write an account of biotechnological applications in medicine.
EASY

Identify the correct sequence of steps that are followed in gene therapy.
A Altered virus is mixed with cells from patient
B. Therapeutic human gene is inserted into the virus (vector)
C. Cells are collected from the patients
D. Cells from the patient become genetically altered
E. Reintroduction of genetically altered cells into patient
F. Suitable virus (vector) is selected and altered
G The genetically altered cells produce the desired proteins or hormones