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Explain how plasmids are used as vectors in genetic engineering.

Important Questions on Biotechnology - Principles and Processes

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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Write down the functions of restriction enzyme and ligase enzyme. 
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Commonly used vectors for human genome sequencing are:
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Enzyme which joins two ends of DNA strand is 
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Examine the diagram and identify the correction option.

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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The DNA fragments separated on an agarose gel can be visualised after staining with:
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The construction of the first recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which vector can clone only a small fragment of DNA?
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

A gene is inserted into the PvuII site of the cloning vector (given below) and transformed into E. coli cells. Which one of the following statements is then true?

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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
A gene whose expression helps to identify transformed cell is known as
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which one of the following represents a palindromic sequence in DNA?
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Match the organism with its use in biotechnology.

(a) Bacillus thuringiensis (i) Cloning vector
(b) Thermus aquaticus (ii) Construction of first rDNA molecule
(c) Agrobacterium tumefaciens (iii) DNA polymerase
(d) Salmonella typhimurium (iv) Cry proteins
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The cutting of DNA at a specific location became possible with the discovery of:
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The ratio of genes for protein involved in plasmid replication rop codes for proteins, Ori site, restriction sites, antibiotic resistance genes present in E. coli cloning vector pBR322 is
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Discuss the importance of cloning vectors in biotechnology.
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Rop-gene which codes the proteins involved in the replication of the plasmid pBR322 in E. coli is located at the restriction site of:
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Identify the correct statements.
(A) In gel electrophoresis, DNA fragments resolve according to their size.
(B) It is not necessary to digest the bacterial cells with lysozyme to isolate DNA
(C) In PCR, DNA polymerase extends the primers using the nucleotides.
(D) During the isolation of DNA, ribonuclease is added along with protease
(E) Agarose gel electrophoresis is employed to check the progression of restriction enzyme digestion.
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

The given figure is the diagrammatic representation of the E. Coli vector pBR 322. Which one of the given options correctly identifies its certain components?

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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Following statements describe the characteristics of restriction endonucleases. Identify the incorrect statement.

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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which of the following is a restriction endonuclease?