MEDIUM
Upper Secondary: IGCSE
IMPORTANT
Earn 100

Explain in your own words how paper chromatography works.

Important Questions on Separating Substances

MEDIUM
Upper Secondary: IGCSE
IMPORTANT
What do you think a locating agent is? 
MEDIUM
Upper Secondary: IGCSE
IMPORTANT
Which chemical you will need, in an experiment to separate amino acids by chromatography?
MEDIUM
Upper Secondary: IGCSE
IMPORTANT
What makes Rfvalues so useful?
MEDIUM
Upper Secondary: IGCSE
IMPORTANT

For the chromatogram above:

Question Image

Were any of the amino acids in B-E also present in A? How can you tell at a glance? 

HARD
Upper Secondary: IGCSE
IMPORTANT

For the chromatogram above:

Question Image

Amino acids Distance travelled (cm)
A 2.5
B 1.5
C 2.5
D 1.5
E 2.1

(solvent front travelled= 3.5)

The amino acids travelled the distance from A-E is given, work out the Rfvalues for the amino acids in A-E.

HARD
Upper Secondary: IGCSE
IMPORTANT
Amino acids Distance travelled (cm) Rfvalues
A 2.5 0.7
B 1.5 0.42
C 2.5 0.7
D 1.5 0.42
E 2.1 0.6

Question Image

Use the Rftable above to name the amino acids. (The Rf values of some amino acids are given)