EASY
Earn 100

Explain the process of horizontal gene transfer in bacteria.

Important Questions on Molecular Genetics

MEDIUM
Biology>Genetics and Evolution>Molecular Genetics>Replication, Transcription, Translation
Which among the following is a selectable marker in pBR – 322 ?
HARD
Biology>Genetics and Evolution>Molecular Genetics>Replication, Transcription, Translation
Give an account of the Blue-White Method of selection of recombinants.
HARD
Biology>Genetics and Evolution>Molecular Genetics>Replication, Transcription, Translation
By particles of which metal recombinant DNA is coated in particle bombardment?
MEDIUM
Biology>Genetics and Evolution>Molecular Genetics>Replication, Transcription, Translation
Explain any two methods for introduction of recombinant DNA. into host cell.
EASY
Biology>Genetics and Evolution>Molecular Genetics>Replication, Transcription, Translation
109 bacteria were spread on an agar plate containing penicillin. After incubation overnight at 37oC 10 bacterial colonies were observed on the plate. That the colonies are likely to be resistant to penicillin can be tested by
EASY
Biology>Genetics and Evolution>Molecular Genetics>Replication, Transcription, Translation
Write the methods to introduce alien DNA into Host cells.
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Biology>Genetics and Evolution>Molecular Genetics>Replication, Transcription, Translation

Write short note on the following :

Gene gun

EASY
Biology>Genetics and Evolution>Molecular Genetics>Replication, Transcription, Translation
Spliceosomes are not found in cells of:
EASY
Biology>Genetics and Evolution>Molecular Genetics>Replication, Transcription, Translation
In biotechnology applications, a selectable marker is incorporated in a plasmid
HARD
Biology>Genetics and Evolution>Molecular Genetics>Replication, Transcription, Translation
Describe two vector less methods of gene transfer used in rDNA technology.
MEDIUM
Biology>Genetics and Evolution>Molecular Genetics>Replication, Transcription, Translation
Choose the wrong statement from the following:
EASY
Biology>Genetics and Evolution>Molecular Genetics>Replication, Transcription, Translation
A gene whose expression helps to identify transformed cell is known as
EASY
Biology>Genetics and Evolution>Molecular Genetics>Replication, Transcription, Translation
An antibiotic resistance gene in a vector usually helps in the selection of
MEDIUM
Biology>Genetics and Evolution>Molecular Genetics>Replication, Transcription, Translation
What is gene-gun?
EASY
Biology>Genetics and Evolution>Molecular Genetics>Replication, Transcription, Translation
The equivalent of a structural gene is:
MEDIUM
Biology>Genetics and Evolution>Molecular Genetics>Replication, Transcription, Translation
Choose the correct pair from the following.
HARD
Biology>Genetics and Evolution>Molecular Genetics>Replication, Transcription, Translation
Which one of the following is wrongly matched?
MEDIUM
Biology>Genetics and Evolution>Molecular Genetics>Replication, Transcription, Translation

Assertion (A): Chromogenic substrates give blue-coloured colonies if the plasmid does not have an insert.

Reason (R): Recombinant colonies produce colour in presence of the chromogenic substrates due to the presence of β galactosidase.
The correct option among the following is:

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Biology>Genetics and Evolution>Molecular Genetics>Replication, Transcription, Translation
In cloning vectors, antibiotic resistance genes are helpful for.
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Biology>Genetics and Evolution>Molecular Genetics>Replication, Transcription, Translation
The technique of bombarding plant cells with high velocity microparticle's of gold or tungsten, coated with DNA, is