EASY
Earn 100

Explain the role of reverse transcriptase in the formation of cDNA.

Important Questions on Genetic Technology

HARD
Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
Explain the mechanism of the action of restriction endonucleases that makes them suitable for genetic engineering.
EASY
Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
The first human hormone produced by recombinant DNA technology is:
HARD
Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
What are restriction endonucleases? Give the rules of their nomenclature.
EASY
Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
Restriction enzymes are used to cut 
MEDIUM
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There is a restriction endonuclease called EcoRI. What does "co" part in it stand for?  
EASY
Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
Recombinant colonies in insertional in activation are differentiated on the basis of
EASY
Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist

Fill in the blanks with the correct options given in the bracket.

Chemical knives of DNA are _____. (Polymerase, Transcriptases, Ligases, Endonucleases)

MEDIUM
Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist

Given below is a sample of a portion of DNA strand giving the base sequence on the opposite strands. What is so special shown in it?

5‘ ----GAATTC----3’
3’ ----CTTAAG-----5’

MEDIUM
Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
Class II restriction endonucleases (enzymes) recognize specific nucleotide sequences in DNA called _____.
EASY
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In insertional inactivation the recombinant DNA is inserted within the coding sequence of
EASY
Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
Which of the following statements does not hold true for restriction enzyme?
EASY
Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
In recombinant DNA technique, the term vector refers to 
EASY
Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
The gene expression like in lac operon is controlled by a _____.
MEDIUM
Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
GAATTC is the recognition site for which of the following restriction endonuclease?
EASY
Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist

Fill in the blanks with the correct options given in the bracket.

The enzyme useful for genetic engineering is _____. (Ligase, Endonuclease, Polymerase, Restriction endonuclease)

EASY
Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
The cDNA is referred to double-stranded _____ DNA.
MEDIUM
Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist

Which of the following are correct statements?

  1. The polyethylene glycol method is used for gene transfer without vector.
  2. The biolistic gun is a suitable method for transferring genes into an animal cell.
  3. The plasmid is a single-stranded DNA.
  4. In recombinant DNA technology cDNA is prepared from mRNA.
EASY
Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
The method(s) that is/are used to differentiate recombinant and non-recombinants is/are
EASY
Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
Restriction endonucleases are obtained from _____.
EASY
Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist

Fill in the blanks with the correct options given in the bracket.

Restriction enzymes may be obtained from _____. (Bacteriophage, Bacterial cells, Plasmids, All prokaryotic cells)