
For each substrate concentration tested, the rate should be measured as soon as possible. Explain why.

Important Questions on Enzymes
Outline an investigation you could carry out to compare the temperature at which the enzyme lactase is completely denatured within ten minutes
When free in solution

Outline an investigation you could carry out to compare the temperature at which the enzyme lactase is completely denatured within ten minutes,
When immobilised in alginate beads

Outline an experiment you could carry out to investigate how long it takes the enzyme lactase to denature at 90°C
When free in solution

Outline an experiment you could carry out to investigate how long it takes the enzyme lactase to denature at 90°C
When immobilised in alginate beads.

Outline how you would determine the optimum pH of the enzyme lactase
When free in solution

Outline how you would determine the optimum pH of the enzyme lactase
When immobilised in alginate beads.



