Choose the wrong statement from the following:

Normally 'Biolistics' method is used to transfer RNA into animal cells

For the construction of rDNA the vector and source DNA should be cut by using same restriction enzyme

Plasmids have the ability to replicate independently within the bacterial cell

In normal conditions DNA cannot pass through cell membrane

The construction of first artificial recombinant DNA molecule was by:

Stanley Cohen and Herbert Boyer using the plasmid of Salmonella typhimurium

Stanley Cohen and Herbert Boyer using the plasmid of Escherichia coli

Arber, Smith and Nathans using the plasmid of Salmonella typhimurium

Arber, Smith and Nathans using the plasmid of Agrobacterium tumefaciens

Restriction endonuclease breaks bond between the:

Sugar-phosphate backbone of nucleic acid

N-glycosidic bond between sugar and base

Biolistics (gene-gun) is suitable for

Constructing recombinant DNA by joining with vectors

Which one of the following represents a palindromic sequence in DNA?

5' - GAATTC - 3' 3' - CTTAAG - 5'

5' - CCAATG - 3' 3' - GAATCC - 5'

5' - CATTAG - 3' 3' - GATAAC - 5'

5' - GATACC - 3' 3' - CCTAAG - 5'

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 1>Q 103

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Earn 100

Genetic engineering is possible because

100% studentsanswered this correctly

Important Questions on Biotechnology - Principles and Processes

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 1>Q 104

Bioreactors can be thought of as vessels in which

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 1>Q 105

The nucleic acid extracted from the animal liver is loaded and run on an agarose gel. After staining, it shows the following pattern:

Question Image

If the remaining sample is treated with $RNAse$ and loaded in gel what result would you expect?

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 1>Q 106

"The integration of natural science and organisms, cells, parts thereof, and molecular analogues for products and services". This definition of biotechnology was given by:

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 1>Q 107

'Molecular scissors' refers to?

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 1>Q 108

In recombinant DNA technology, the term vector refers to:

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 1>Q 109

The term 'recombinant DNA' refers to

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 1>Q 110

Which of the following contains the key tools for recombinant DNA technology?
(i) Restriction endonucleases, ligases, vectors
(ii) Ligases, host organism, polymerase enzymes
(iii) Vectors, Taq polymerase, primers
(iv) Restriction exonucleases, ligases, primers, bioreactors

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 1>Q 111

An enzyme catalysing the removal of nucleotides from the ends of