H. Boyer S. Cohen are famous for their research work on
Important Questions on Principles and Processes of Biotechnology
Assertion (A): The fragments of DNA generated by the action of restriction endonucleases are separated by polyacrylamide gel electrophoresis.
Reason (R)： The separated DNA fragments are visualised as bright orange coloured bands after staining with Ethidium Bromide followed by exposure to - radiation.
Which of the following is true?
Match the following lists.
| List I | List II |
| A) Remove nucleotides from the end of the DNA | I) Polymerases |
| B) Restriction enzyme | II) Endonuclease |
| C) Enzyme which cuts at specific position of DNA | III) Ligase |
| D) Stick the complementary cut ends of DNA with H2 bond | IV) Nucleases |
| V) Exonucleases |
The correct match is:
I) More bubble formation
II) Decrease the surface area for transfer
III) It has foam control system
Which DNA fragment of a vector that helps in identifying and eliminating non-transformant cells and permit the growth of transformant cells?
Assertion (A): Chromogenic substrates give blue-coloured colonies if the plasmid does not have an insert.
Reason (R): Recombinant colonies produce colour in presence of the chromogenic substrates due to the presence of galactosidase.
The correct option among the following is:
(A) In gel electrophoresis, DNA fragments resolve according to their size.
(B) It is not necessary to digest the bacterial cells with lysozyme to isolate DNA
(C) In PCR, DNA polymerase extends the primers using the nucleotides.
(D) During the isolation of DNA, ribonuclease is added along with protease
(E) Agarose gel electrophoresis is employed to check the progression of restriction enzyme digestion.
