Choose the wrong statement from the following:

Normally 'Biolistics' method is used to transfer RNA into animal cells

For the construction of rDNA the vector and source DNA should be cut by using same restriction enzyme

Plasmids have the ability to replicate independently within the bacterial cell

In normal conditions DNA cannot pass through cell membrane

The construction of first artificial recombinant DNA molecule was by:

Stanley Cohen and Herbert Boyer using the plasmid of Salmonella typhimurium

Stanley Cohen and Herbert Boyer using the plasmid of Escherichia coli

Arber, Smith and Nathans using the plasmid of Salmonella typhimurium

Arber, Smith and Nathans using the plasmid of Agrobacterium tumefaciens

Restriction endonuclease breaks bond between the:

Sugar-phosphate backbone of nucleic acid

N-glycosidic bond between sugar and base

Biolistics (gene-gun) is suitable for

Constructing recombinant DNA by joining with vectors

Which one of the following represents a palindromic sequence in DNA?

5' - GAATTC - 3' 3' - CTTAAG - 5'

5' - CCAATG - 3' 3' - GAATCC - 5'

5' - CATTAG - 3' 3' - GATAAC - 5'

5' - GATACC - 3' 3' - CCTAAG - 5'

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Identify A, B, C and D in the given figure of E. coli cloning vector pBR322 and select the correct option.

A	B	C	D
HindI	EcoRI	amp^R	ori

A	B	C	D
HindI	BamHi	kan^R	amp^R

A	B	C	D
BamHI	PstI	ori	Amp

A	B	C	D
EcoRI	BamHI	amp^R	ori

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Important Questions on Biotechnology - Principles and Processes

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 16

Which of the following is not a vector-less method of gene transfer?

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 17

Read the following statements and select the correct ones.
(i) Electrophoresis is a technique used for the separation of molecules based on their size and charge.
(ii) Plasmids are extra-chromosomal, self-replicating, usually circular, double stranded DNA molecules found naturally in many bacteria and also in some yeast.
(iii) It is not advisable to use an exonuclease enzyme while producing a recombinant DNA molecule.
(iv) In EcoRI, the roman numeral I indicates that it was the first enzyme isolated from E.coli RY 13.

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 18

Which of the following is correct with respect to the movement of DNA fragments on agarose gel electrophoresis?

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 19

Which of the following steps should be performed by a person in order to visualise the bands of DNA fragments obtained from gel electrophoresis?

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 20

Having become an expert on gel electrophoresis, you are asked to examine a gel. Where would you find the smallest segments of DNA?

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 21

The DNA fragments separated on agarose gel can be visualized after staining with:

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 22

Study the given figure carefully and select the incorrect statements regarding this.
Question Image

(i) It represents a typical agarose gel electrophoresis in which lane 1 contains undigested DNA.
(ii) Smallest DNA bands are formed at A and largest DNA bands are formed at B.
(iii) The separated DNA fragments can be visualized after staining in the visible light.
(iv) The separated DNA bands are cut out from the agarose gel and extracted from the gel piece. This step is known as elution.

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 23

Which of the following is the most suitable method of introducing foreign DNA into the plant cell?

Identify A, B, C and D in the given figure of E. coli cloning vector pBR322 and select the correct option.

Important Questions on Biotechnology - Principles and Processes

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