MEDIUM
12th CBSE
IMPORTANT
Earn 100

Illustrate the recognition sequence of EcoRI and mention what such sequences are called.

Important Questions on Biotechnology: Principles and Processes

MEDIUM
12th CBSE
IMPORTANT
How does restriction endonuclease act on a DNA molecule?
MEDIUM
12th CBSE
IMPORTANT
Any recombinant DNA with the desired gene is required in billion copies for commercial use. How is the amplification done? Explain.
MEDIUM
12th CBSE
IMPORTANT
Name the GM bacterium whose product is used as a clot buster. Name the product. Specify its use in medical science.
MEDIUM
12th CBSE
IMPORTANT
Describe the characteristics a cloning vector must possess.
MEDIUM
12th CBSE
IMPORTANT
Why DNA cannot pass through the cell membrane? Explain how is a bacterial cell made competent to take up recombinant DNA from the medium.
MEDIUM
12th CBSE
IMPORTANT
With the help of diagrams, show the different steps in the formation of recombinant DNA by the action of restriction endonuclease enzyme EcoRI.
MEDIUM
12th CBSE
IMPORTANT
Name the technique that is used for separating the fragments of DNA cut by restriction endonucleases.
MEDIUM
12th CBSE
IMPORTANT
How is the amplification of a gene sample of interest carried out using polymerase chain reaction (PCR)?