MEDIUM
Upper Secondary: IGCSE
IMPORTANT
Earn 100

In a chromatography experiment, eight coloured substances were spotted onto a piece of filter paper. Three were the basic coloured red, blue and yellow. The other were unknown substances, labelled A-E. This shows the resulting chromatogram :

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Which contains all three basic colours?

Important Questions on Separating Substances

MEDIUM
Upper Secondary: IGCSE
IMPORTANT

In a chromatography experiment, eight coloured substances were spotted on a piece of filter paper. Three were the basic coloured red, blue and yellow. The other was unknown substances, labelled A-E. This shows the resulting chromatogram :

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The solvent was propanone. Which of the three basic colours are the most soluble in propanone?

MEDIUM
Upper Secondary: IGCSE
IMPORTANT

The diagram below shows a chromatogram for a mixture of amino acids. 

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The solvent was a mixture of water, butanol and ethanoic acid. Using the table of Rfvalues given, identify the two amino acids.

MEDIUM
Upper Secondary: IGCSE
IMPORTANT

The diagram below shows a chromatogram for a mixture of amino acids.

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The solvent was a mixture of water, butanol and ethanoic acid. Which of them is less soluble in the solvent?

MEDIUM
Upper Secondary: IGCSE
IMPORTANT

The diagram below shows a chromatogram for a mixture of amino acids. 

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Amino acid Rf value
Cysteine 0.08
Lysine 0.14
Glycine 0.26
Serine 0.27
Alanine 0.38
Proline 0.43
Valine 0.60
Leucine 0.73

The solvent was a mixture of water, butanol and ethanoic acid. How will the Rfvalues change if the solvent travels only 6cm?

HARD
Upper Secondary: IGCSE
IMPORTANT
You have three colourless solutions. Each contains an amino acid you must identify. Explain how to do this using chromatography. Use the term Rfand locating agent in your answer, and show that you understand what they mean.