
Inactive Bt toxin converted in to active Bt-toxin due to :-

Important Questions on Biotechnology and Its Applications
Assertion (A) : Transgenic Golden Rice is rich in Vitamin A and prevents blindness.
Reason (R) : It is obtained from "Taiwan"





Assertion Bt toxin exists as inactive prototoxin but once an insect ingests the inactive toxin, it is converted into an active form of toxin.
Reason Protoxin gets activated in insect gut due to the acidic in the gut, which solubilises the protein crystals.
The correct answer is:


Assertion A: In Bt cotton B. thuringiensis produces a toxic insecticidal crystalline protein which destroys bollworms.
Reason R: B. thuringiensis produces this toxic protein in an inactive form, but when an insect ingests this inactive protein, it is converted into active form of toxin due to the alkaline pH of gut which solubilises the crystals, which is responsible for the death of bollworm.



Reason (R): and controls the cotton boll worms







Match the organism with its use in biotechnology.
(a) | Bacillus thuringiensis | (i) | Cloning vector |
(b) | Thermus aquaticus | (ii) | Construction of first rDNA molecule |
(c) | Agrobacterium tumefaciens | (iii) | DNA polymerase |
(d) | Salmonella typhimurium | (iv) | Cry proteins |

To conduct detailed study into disease causing organism in livestock, Biosafety laboratory has been setup in India in:
1. Ludhiana
2. Bengaluru
3. Anand
4. Allahabad


