Make a diagram to show how a piece of DNA is cut by restriction enzymes and then inserted into a plasmid. Show both strands of DNA in your diagram.
Important Questions on Genetic Technology
Rearrange the statements below to produce a flow diagram showing the steps involved in producing bacteria capable of synthesising a recombinant human protein, such as insulin or factor VIII.
A. Insert the plasmid into a host bacterium.
B. Isolate mRNA for the gene required.
C. Use ligase to seal the sugar-phosphate backbone of the recombinant plasmid.
D. Use DNA polymerase to produce double-stranded eDNA.
E. Clone the modified bacteria and harvest the recombinant protein.
F. Use reverse transcriptase to produce single-stranded cDNA.
G. Use a restriction enzyme to cut plasmids.
H. Use an enzyme to add a short length of single-stranded DNA to form sticky ends.
I. Form recombinant plasmids by complementary base pairing.
J. Mix the double-stranded DNA with plasmids.
