Match the items given in Column I with their uses in Column II and choose the correct option. Column I Column II A. Escherichia coli i) Thermostable DNA polymerase B. Thermus aquaticus ii) Restriction endonuclease C. Agrobacterium tumefaciens iii) Gene gun D. Biolistic iv) Ti plasmid

A - ii), B - i), C - iv), D - iii)

A - i), B - ii), C - iv), D - iii)

A - iv), B - i), C - iii), D - ii)

A - iii), B - iv), C - ii), D - i)

Match the options given in Column I with Column II and choose the correct option. Column I Column II A. Gene taxi i) Restriction endonuclease B. HindII ii) Vector-less gene transfer C. Gene gun iii) Transposon D. Mobile genetic element iv) Plasmid

A - iv), B - i), C - ii), D - iii)

A - i), B - ii), C - iii), D - iv)

A - ii), B - iv), C - i), D - iii)

A - iv), B - iii), C - ii), D - i)

Choose the wrong statement from the following:

Normally 'Biolistics' method is used to transfer RNA into animal cells

For the construction of rDNA the vector and source DNA should be cut by using same restriction enzyme

Plasmids have the ability to replicate independently within the bacterial cell

In normal conditions DNA cannot pass through cell membrane

The construction of first artificial recombinant DNA molecule was by:

Stanley Cohen and Herbert Boyer using the plasmid of Salmonella typhimurium

Stanley Cohen and Herbert Boyer using the plasmid of Escherichia coli

Arber, Smith and Nathans using the plasmid of Salmonella typhimurium

Arber, Smith and Nathans using the plasmid of Agrobacterium tumefaciens

Restriction endonuclease breaks bond between the:

Sugar-phosphate backbone of nucleic acid

N-glycosidic bond between sugar and base

Biolistics (gene-gun) is suitable for

Constructing recombinant DNA by joining with vectors

Which one of the following represents a palindromic sequence in DNA?

5' - GAATTC - 3' 3' - CTTAAG - 5'

5' - CCAATG - 3' 3' - GAATCC - 5'

5' - CATTAG - 3' 3' - GATAAC - 5'

5' - GATACC - 3' 3' - CCTAAG - 5'

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Match the terms given in Column I with their definitions in Column II and select the correct answer from codes given below.

Column I Column II

$(a)$ Transformation $(i)$ Sequences cut by restriction enzymes

$(b)$ Recognition $(i i)$ Process by which DNA fragments are separated based on their size

$(c)$ Gel electrophoresis $(i i i)$ Plasmid DNA that has incorporated human DNA

$(d)$ Recombinant DNA $(i v)$ Process by which bacteria take up pieces of DNA from the environment

	Column I		Column II
$(a)$	Transformation	$(i)$	Sequences cut by restriction enzymes
$(b)$	Recognition	$(i i)$	Process by which DNA fragments are separated based on their size
$(c)$	Gel electrophoresis	$(i i i)$	Plasmid DNA that has incorporated human DNA
$(d)$	Recombinant DNA	$(i v)$	Process by which bacteria take up pieces of DNA from the environment

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Important Questions on Biotechnology - Principles and Processes

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 3>Q 6

Match the items given in Column I with their uses in Column II and choose the correct option.

Column I	Column II
A. Escherichia coli	i) Thermostable DNA polymerase
B. Thermus aquaticus	ii) Restriction endonuclease
C. Agrobacterium tumefaciens	iii) Gene gun
D. Biolistic	iv) Ti plasmid

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 3>Q 8

Which of the following is not a characteristic feature of the vector pBR322?

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 3>Q 12

If the desired gene segment is inserted at the SalI restriction site in pBR322 plasmid, the resulting plasmid will confer resistance to

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 3>Q 14

Fill up the blanks and select the correct option.
(i) EcoRI cuts the DNA between bases ____ only when the sequence ____ is present in the DNA duplex.
(ii) Disruption of the cell membranes can be achieved by treating the bacterial cells, plant cells and fungal cells with enzymes respectively _____, _____ and_____.
(iii) Since DNA has a _____ charge, it moves towards the _____ of the electrophoretic chamber.

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 3>Q 17

Read the given statements and select the correct option.
Statement 1: In insertional inactivation, blue colour produced by bacterial colonies indicates that the plasmid does not have an insert into the bacterial genome.
Statement 2: Presence of insert results into insertional inactivation of

β

-galactosidase enzyme and the colonies do not produce any colour.

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 3>Q 13

Blunt or flush ends are produced by all the following, except

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 3>Q 19

Match the options given in Column I with Column II and choose the correct option.

Column I	Column II
A. Gene taxi	i) Restriction endonuclease
B. HindII	ii) Vector-less gene transfer
C. Gene gun	iii) Transposon
D. Mobile genetic element	iv) Plasmid

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 3>Q 21

Match Column I (enzyme) with Column II (characteristics/ activity) and select the correct answer from the given codes.

	Column I		Column II
$(a)$	Taq DNA polymerase	$(i)$	Cleaves the ends of linear DNA
$(b)$	Exonuclease	$(i i)$	Breakdown of fungal cell wall
$(c)$	Protease	$(i i i)$	Stable above $90^{o}$ C
$(d)$	Chitinase	$(i v)$	Made only by eukaryotic cells
		$(v)$	Degradation of proteins

Important Questions on Biotechnology - Principles and Processes

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