MEDIUM
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Molecular scissors or biological scissors refer to
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Important Questions on Biotechnology - Principles and Processes
EASY
The ratio of genes for protein involved in plasmid replication rop codes for proteins, Ori site, restriction sites, antibiotic resistance genes present in E. coli cloning vector is
EASY
Which vector can clone only a small fragment of DNA?
EASY
plasmid is obtained from ___.
HARD
What is endonuclease? What do you mean by restriction site?
HARD
Explain the mechanism of the action of restriction endonucleases that makes them suitable for genetic engineering.
HARD
What do you mean by cDNA. What is transfection?
MEDIUM
Match the organism with its use in biotechnology.
| (a) | Bacillus thuringiensis | (i) | Cloning vector |
| (b) | Thermus aquaticus | (ii) | Construction of first rDNA molecule |
| (c) | Agrobacterium tumefaciens | (iii) | DNA polymerase |
| (d) | Salmonella typhimurium | (iv) | Cry proteins |
EASY
Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes?
HARD
Give an account of the Blue-White Method of selection of recombinants.
EASY
Commonly used vectors for human genome sequencing are:
HARD
Which one of the following is an example of restriction endonuclease enzyme?
HARD
What are restriction endonucleases? Give the rules of their nomenclature.
HARD
The cloning vector PBR322 used in genetic engineering is a/an
HARD
In biotechnology, which enzymes are called ‘molecular scissors’ and ‘molecular glue’?
HARD
Describe the essential steps in genetic engineering.
EASY
The cutting of DNA at a specific location became possible with the discovery of:
EASY
Which of the following is a restriction endonuclease?
EASY
DNA's precipitation out of a mixture of biomolecules can be achieved by treatment with:
MEDIUM
Explain what are the desired characteristics of an ideal cloning vector used in rDNA technology.
HARD
Describe two vector less methods of gene transfer used in rDNA technology.
