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Earn 100

Nidhi performed gel electrophoresis after treating one vector with restriction enzymes. She added one mixture in well Q and another mixture in well R. Given below is an image of the results. 

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What is the likely reason that the fragments in wells Q and R are different? 

Important Questions on Biotechnology - Principles and Processes

EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Identify the palindromic sequence.
HARD
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Eco RI and Rsa I restriction endonucleases require 6bp and 4bp sequences respectively for cleavage. In a 10 kb DNA fragment how many probable cleavage sites are present for these enzymes
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Following statements describe the characteristics of restriction endonucleases. Identify the incorrect statement.

EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Select the correct statement from the following:
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Restriction enzyme work by cleaving
MEDIUM
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Identify the wrong statement with regard to restriction enzymes.
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which one of the following represents a palindromic sequence in DNA?
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Identify the palindromic sequence in the following base sequences
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
How many linear DNA fragments will be produced when a circular plasmid is digested with a restriction enzyme having 3 sites?
HARD
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
A scientist has cloned an 8 Kb fragment of a mouse gene into the Eco RI site of a vector of 6 Kb size. The cloned DNA has no other Eco RI site within. Digestions of the cloned DNA is shown below

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Which one of the following sets of DNA fragments generated by digestion with both Eco RI and Bam HI as shown in (iii) is from the gene?
MEDIUM
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
In a restriction enzyme digestion experiment, a DNA fragment of 1 kilobases length is used. This fragment harbors three 5'-GAATTC-3' restriction sites. After the restriction digestion of this fragment with EcoRI, 3 DNA fragments corresponding to 500, 300 & 100 base pairs are observed in the agarose gel electrophoresis AGE. Predict the positions of the restriction site (GAATTC) in the undigested 1 kb-fragment which will result in the observed bands in AGE.
MEDIUM
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which ONE of the following statements is INCORRECT about restriction endonucleases?
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Study the following lists:

  List-I   List-II
A. Eco RI I. Vector for transgenic plants
B. Ti plasmid II. Restriction enzyme
C. pBR 322 III. Probe
D. Reverse transcriptase IV. RNA dependent DNA synthesis
    V. Artificial plasmid

The correct match is

EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Genomic DNA is digested with Alu I, a restriction enzyme which is a four base-pair cutter. What is the frequency with which it will cut the DNA assuming a random distribution of bases in the genome?
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Match the following lists:

  List I   List II
A) Hind II I) pBR 322
B) Eco RI II) Replication of the plasmid
C) Hind III III) First restriction endonuclease
D) rop codes IV) E. coli Ry 13

The correct match is

MEDIUM
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Identify the DNA sequence which can be cut using EcoRI.
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Choose the bacterium which is not a source of REN:
HARD
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
A circular plasmid of 10,000 base pairs (bp) is digested with two restriction enzymes, A and B, to produce 3000 bp and 2000 bp bands when visualised on an agarose gel. When digested with one enzyme at a time, only one band is visible at 5000 bp. If the first site for enzyme A (A1) is present at the 100th base, the order in which the remaining sites (A2, B1, and B2) are present is:
MEDIUM
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Consider the linear double-stranded DNA shown below. The restriction enzyme sites and the lengths demarcated are shown. This DNA is completely digested with both Eco RI and Bam HI restriction enzymes. If the product is analyzed by gel electrophoresis, how many distinct bands would be observed?

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EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which of the following is a restriction endonuclease?