
Outline an experiment you could carry out to investigate how long it takes the enzyme lactase to denature at 90°C
When immobilised in alginate beads.
Important Questions on Enzymes
Outline how you would determine the optimum pH of the enzyme lactase
When free in solution

Outline how you would determine the optimum pH of the enzyme lactase
When immobilised in alginate beads.





The graph shows the progress of the digestion of starch by the enzyme salivary amylase. Why does the reaction slow down?


If methylene blue dye is added to a suspension of yeast cells, any living cells remain colourless. However, any dead cells are stained blue. This fact was used to carry out an investigation into the rate at which yeast cells were killed at two different temperatures (at high temperatures the yeast enzymes will be denatured). The results are shown in the diagram.
