Match the following techniques or instruments with their usage: (a) Bioreactor (i) Separation of DNA fragments (b) Electrophoresis (ii) Production of large quantities of products (c) PCR (iii) Detection of pathogen, based on antigen-antibody reaction (d) ELISA (iv) Amplification of nucleic acids Select the correct option from following:

( a ) - ( ii ) , ( b ) - ( i ) , ( c ) - ( iv ) , ( d ) - ( iii )

( a ) - ( iii ) , ( b ) - ( ii ) , ( c ) - ( iv ) , ( d ) - ( i )

( a ) - ( iv ) , ( b ) - ( iii ) , ( c ) - ( ii ) , ( d ) - ( i )

( a ) - ( ii ) , ( b ) - ( i ) , ( c ) - ( iii ) , ( d ) - ( iv )

HARD

Earn 100

Read the following sentences:-
(a) In elution, the separated bands of DNA are cut out from agarose gel & extracted from the gel piece.
(b) E. coli cloning vector pBR322 has several restriction sites, Ori, antibiotic resistance genes & ROP.
(c) The downstream processing and quality control testing vary from product to product.
(d) Competent bacterial cells cannot take up the plasmid.

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Important Questions on Biotechnology - Principles and Processes

MEDIUM

Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Match the following techniques or instruments with their usage:

(a)	Bioreactor	(i)	Separation of DNA fragments
(b)	Electrophoresis	(ii)	Production of large quantities of products
(c)	$PCR$	(iii)	Detection of pathogen, based on antigen-antibody reaction
(d)	$ELISA$	(iv)	Amplification of nucleic acids

Select the correct option from following:

EASY

Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

The Taq polymerase enzyme is obtained from:

EASY

Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

To obtain reliable results from PCR machine, the process can be carried out for about ______ cycle.

EASY

Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?

EASY

Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Stirred-tank bioreactors have been designed for:

EASY

Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Taq polymerase that finds its application in PCR is obtained from:

MEDIUM

Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Which one among the following primer pairs would amplify the fragment of $DNA$ given below?

$5^{'} - CTAGTCGTCGAT - {(N)}_{300} - GACTGAGCTGAGCTG - 3'$

$3^{'} \cdot GATCAGCAGCTA - (N)_{300} - CTGACTCGACTCGAC - 5^{'}$

EASY

Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Which of the following is not required for any of the techniques of DNA fingerprinting available at present?

HARD

Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Which of the following is not a component of downstream processing?

EASY

Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

From the given combinations of steps in PCR, identify the enzyme dependent step/s.

EASY

Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?

MEDIUM

Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Select the correct steps involved in amplification of gene

EASY

Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Which of the following is not an application of PCR (Polymerase Chain Reaction)?

EASY

Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Rapid antigen test and RT-PCR are the two diagnostic tests for Covid

19

virus. PCR is a molecular diagnostic tool, that stands for:

HARD

Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

The correct order of steps in Polymerase Chain Reaction (PCR) is

EASY

Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

The DNA fragments separated on an agarose gel can be visualised after staining with:

EASY

Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

What is the following is correct for the movement of DNA fragments on agarose gel during gel electrophoresis?

EASY

Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Suppose DNA samples collected for DNA fingerprinting analysis are less than the required quantity. Which of the following techniques is helpful to make the samples sufficient for above analysis?

EASY

Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Nowadays, the early diagnosis of bacterial or viral infection in humans is possible using

EASY

Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

The process of separation and purification of expressed protein before marketing is called

Important Questions on Biotechnology - Principles and Processes

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