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Earn 100

Read the following statements and select the correct ones.
(i) Same kind of sticky ends are produced when a DNA has been cut by different restriction enzymes.
(ii) Exonucleases make cuts at specific positions within the DNA.
(iii) Hind II was the first restriction endonuclease to be isolated.
(iv) A bacteriophage has the ability to replicate within bacterial cells by integrating its DNA with bacterial DNA.
(v) Presence of more than one recognition sites for an enzyme within the vector complicates the gene cloning.

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Important Questions on Biotechnology - Principles and Processes

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Plasmids are ideal vectors for gene cloning as they:
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Which of the following is not a source of restriction endonuclease?
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Which of the following is an incorrect statement?
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The enzyme used to combine two fragments of DNA is:
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The role of DNA ligase in the construction of recombinant DNA molecule is:
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The specific DNA sequence where EcoRI cuts is:
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Match Column I with Column II and select the correct answer from the given codes.
  Column I   Column II
(a) ampR gene (i) Artificial plasmid
(b) Separation of DNA fragments (ii) Selectable marker
(c) Hindlll (iii) Electrophoresis
(d) pBR322 (iv) Haemophilus influenzae
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How are transformants selected from non-transformants?