MEDIUM
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Restriction endonuclease enzyme is also called molecular scissors because they cut the hydrogen bond at specific site of DNA.
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Important Questions on Principles and Process of Biotechnology
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plasmid is obtained from ___.
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Which vector can clone only a small fragment of DNA?
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Identify the wrong statement with regard to restriction enzymes.
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Restriction endonuclease recognises palindromic nucleotide sequences, because they have characteristic
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What is the following is correct for the movement of DNA fragments on agarose gel during gel electrophoresis?
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Identify the labels and in the following Agarose gel electrophoresis representation.
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In gel electrophoresis, separated DNA fragments can be visualized with the help of
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The ratio of genes for protein involved in plasmid replication rop codes for proteins, Ori site, restriction sites, antibiotic resistance genes present in E. coli cloning vector is
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The DNA fragments separated on an agarose gel can be visualised after staining with:
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Following statements describe the characteristics of restriction endonucleases. Identify the incorrect statement.
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Assertion (A): The fragments of DNA generated by the action of restriction endonucleases are separated by polyacrylamide gel electrophoresis.
Reason (R)： The separated DNA fragments are visualised as bright orange coloured bands after staining with Ethidium Bromide followed by exposure to - radiation.
Which of the following is true?
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The cutting of DNA at a specific location became possible with the discovery of:
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In a restriction enzyme digestion experiment, a fragment of kilobases length is used. This fragment harbors three restriction sites. After the restriction digestion of this fragment with EcoRI, fragments corresponding to base pairs are observed in the agarose gel electrophoresis . Predict the positions of the restriction site in the undigested -fragment which will result in the observed bands in .
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Biolistics method is suitable for gene transfer into ___.
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Sequence of strategies involved for the isolation of gene of interest from a plant cell during the process of rDNA technology
Dissolution of biological membranes
Enzymatic digestion of cell wall
Precipitation of DNA
Removal of DNA by spooling
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Match the following lists:
| List | List | ||
| A) | Hind | pBR | |
| B) | Eco RI | Replication of the plasmid | |
| C) | Hind | First restriction endonuclease | |
| D) | rop codes | E. coli Ry |
The correct match is
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Study the following lists:
| List- | List- | ||
| A. | Eco RI | Vector for transgenic plants | |
| B. | Ti plasmid | Restriction enzyme | |
| C. | pBR | Probe | |
| D. | Reverse transcriptase | RNA dependent DNA synthesis | |
| Artificial plasmid |
The correct match is
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Commonly used vectors for human genome sequencing are:
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A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using
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Which of the following is a restriction endonuclease?
