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Restriction endonuclease is an enzyme commonly known as genetic glue.

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Important Questions on Genetics of the Future

EASY
Science>Biology>Genetics of the Future>Genetic Engineering
Identify the palindromic sequence.
HARD
Science>Biology>Genetics of the Future>Genetic Engineering
A circular plasmid of 10,000 base pairs (bp) is digested with two restriction enzymes, A and B, to produce 3000 bp and 2000 bp bands when visualised on an agarose gel. When digested with one enzyme at a time, only one band is visible at 5000 bp. If the first site for enzyme A (A1) is present at the 100th base, the order in which the remaining sites (A2, B1, and B2) are present is:
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Science>Biology>Genetics of the Future>Genetic Engineering

Following statements describe the characteristics of restriction endonucleases. Identify the incorrect statement.

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Science>Biology>Genetics of the Future>Genetic Engineering
Select the correct statement from the following:
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Science>Biology>Genetics of the Future>Genetic Engineering
Restriction enzyme work by cleaving
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Science>Biology>Genetics of the Future>Genetic Engineering
Identify the wrong statement with regard to restriction enzymes.
EASY
Science>Biology>Genetics of the Future>Genetic Engineering
Which one of the following represents a palindromic sequence in DNA?
EASY
Science>Biology>Genetics of the Future>Genetic Engineering
Identify the palindromic sequence in the following base sequences
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Science>Biology>Genetics of the Future>Genetic Engineering
How many linear DNA fragments will be produced when a circular plasmid is digested with a restriction enzyme having 3 sites?
HARD
Science>Biology>Genetics of the Future>Genetic Engineering
A scientist has cloned an 8 Kb fragment of a mouse gene into the Eco RI site of a vector of 6 Kb size. The cloned DNA has no other Eco RI site within. Digestions of the cloned DNA is shown below

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Which one of the following sets of DNA fragments generated by digestion with both Eco RI and Bam HI as shown in (iii) is from the gene?
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In a restriction enzyme digestion experiment, a DNA fragment of 1 kilobases length is used. This fragment harbors three 5'-GAATTC-3' restriction sites. After the restriction digestion of this fragment with EcoRI, 3 DNA fragments corresponding to 500, 300 & 100 base pairs are observed in the agarose gel electrophoresis AGE. Predict the positions of the restriction site (GAATTC) in the undigested 1 kb-fragment which will result in the observed bands in AGE.
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Which ONE of the following statements is INCORRECT about restriction endonucleases?
EASY
Science>Biology>Genetics of the Future>Genetic Engineering

Study the following lists:

  List-I   List-II
A. Eco RI I. Vector for transgenic plants
B. Ti plasmid II. Restriction enzyme
C. pBR 322 III. Probe
D. Reverse transcriptase IV. RNA dependent DNA synthesis
    V. Artificial plasmid

The correct match is

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Science>Biology>Genetics of the Future>Genetic Engineering
Genomic DNA is digested with Alu I, a restriction enzyme which is a four base-pair cutter. What is the frequency with which it will cut the DNA assuming a random distribution of bases in the genome?
EASY
Science>Biology>Genetics of the Future>Genetic Engineering

Match the following lists:

  List I   List II
A) Hind II I) pBR 322
B) Eco RI II) Replication of the plasmid
C) Hind III III) First restriction endonuclease
D) rop codes IV) E. coli Ry 13

The correct match is

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Science>Biology>Genetics of the Future>Genetic Engineering
Identify the DNA sequence which can be cut using EcoRI.
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Science>Biology>Genetics of the Future>Genetic Engineering
Choose the bacterium which is not a source of REN:
HARD
Science>Biology>Genetics of the Future>Genetic Engineering
Eco RI and Rsa I restriction endonucleases require 6bp and 4bp sequences respectively for cleavage. In a 10 kb DNA fragment how many probable cleavage sites are present for these enzymes
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Science>Biology>Genetics of the Future>Genetic Engineering

Consider the linear double-stranded DNA shown below. The restriction enzyme sites and the lengths demarcated are shown. This DNA is completely digested with both Eco RI and Bam HI restriction enzymes. If the product is analyzed by gel electrophoresis, how many distinct bands would be observed?

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EASY
Science>Biology>Genetics of the Future>Genetic Engineering
Which of the following is a restriction endonuclease?