Choose the wrong statement from the following:

Normally 'Biolistics' method is used to transfer RNA into animal cells

For the construction of rDNA the vector and source DNA should be cut by using same restriction enzyme

Plasmids have the ability to replicate independently within the bacterial cell

In normal conditions DNA cannot pass through cell membrane

The construction of first artificial recombinant DNA molecule was by:

Stanley Cohen and Herbert Boyer using the plasmid of Salmonella typhimurium

Stanley Cohen and Herbert Boyer using the plasmid of Escherichia coli

Arber, Smith and Nathans using the plasmid of Salmonella typhimurium

Arber, Smith and Nathans using the plasmid of Agrobacterium tumefaciens

Restriction endonuclease breaks bond between the:

Sugar-phosphate backbone of nucleic acid

N-glycosidic bond between sugar and base

Biolistics (gene-gun) is suitable for

Constructing recombinant DNA by joining with vectors

Which one of the following represents a palindromic sequence in DNA?

5' - GAATTC - 3' 3' - CTTAAG - 5'

5' - CCAATG - 3' 3' - GAATCC - 5'

5' - CATTAG - 3' 3' - GATAAC - 5'

5' - GATACC - 3' 3' - CCTAAG - 5'

E M B I B E

Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 30

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Restriction endonucleases are utilised in genetic engineering as

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Important Questions on Biotechnology - Principles and Processes

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 31

Which of the following is an incorrect statement?

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 32

In a polymerase chain reaction, temperature required for the steps, respectively
(i) Denaturation,
(ii) Annealing and
(iii) Extension

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 33

Multiple cloning sites (MCS) in an ideal vector

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 34

All of the following are characteristics of an ideal vector, except

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 35

Which of the following statements are correct for the enzyme Taq polymerase?
(i) It remains active during the high temperature induced denaturation of dsDNA.
(ii) It requires primers for carrying out the process of polymerisation.
(iii) It synthesises the RNA region between the primers, using dNTPs and Mg²⁺.

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 36

Which of the following is an incorrect statement regarding the enzymes used for recombinant DNA technology?

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 37

The flow chart given below represents the process of recombinant DNA technology. Identify A, B, C and D.
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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 38

How many fragments will be generated on the digestion of a closed circular DNA molecule with a restriction enzyme having six recognition sites on the DNA?

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