Study the given data and answer the following question.
A sample of an enzyme called lactase was isolated from the intestinal lining of a calf. Assays were undertaken to evaluate the activity of the enzyme sample. The substrate of lactase is the disaccharide lactose. Lactase breaks a lactose molecule in two, producing a glucose molecule and a galactose molecule.
Two assays were carried out.
Assay 1:
Lactose concentration (% w/v)
15
15
15
15
15
15
Concentration of enzyme sample (% v/v)
0
5
10
15
20
25
Reaction rate glucose sec-1 mL-1
0
25
50
75
100
125
Assay 2:
Lactose concentration (% w/v)
0
5
15
20
25
30
Concentration of enzyme sample (% v/v)
5
5
5
5
5
5
Reaction rate glucose sec-1 mL-1
0
15
25
35
40
40
What are the variables in each of the two assays?
Important Questions on Biomolecules
Study the given data and answer the following question.
A sample of an enzyme called lactase was isolated from the intestinal lining of a calf. Assays were undertaken to evaluate the activity of the enzyme sample. The substrate of lactase is the disaccharide lactose. Lactase breaks a lactose molecule in two, producing a glucose molecule and a galactose molecule.
Two assays were carried out.
Assay 1:
| Lactose concentration (% w/v) | 15 | 15 | 15 | 15 | 15 | 15 |
| Concentration of enzyme sample (% v/v) | 0 | 5 | 10 | 15 | 20 | 25 |
| Reaction rate glucose sec-1 mL-1 | 0 | 25 | 50 | 75 | 100 | 125 |
Assay 2:
| Lactose concentration (% w/v) | 0 | 5 | 15 | 20 | 25 | 30 |
| Concentration of enzyme sample (% v/v) | 5 | 5 | 5 | 5 | 5 | 5 |
| Reaction rate glucose sec-1 mL-1 | 0 | 15 | 25 | 35 | 40 | 40 |
Which of the following statements can be concluded from the two assays?
Choose a correct answer from the following.
