Choose the wrong statement from the following:

Normally 'Biolistics' method is used to transfer RNA into animal cells

For the construction of rDNA the vector and source DNA should be cut by using same restriction enzyme

Plasmids have the ability to replicate independently within the bacterial cell

In normal conditions DNA cannot pass through cell membrane

The construction of first artificial recombinant DNA molecule was by:

Stanley Cohen and Herbert Boyer using the plasmid of Salmonella typhimurium

Stanley Cohen and Herbert Boyer using the plasmid of Escherichia coli

Arber, Smith and Nathans using the plasmid of Salmonella typhimurium

Arber, Smith and Nathans using the plasmid of Agrobacterium tumefaciens

Restriction endonuclease breaks bond between the:

Sugar-phosphate backbone of nucleic acid

N-glycosidic bond between sugar and base

Biolistics (gene-gun) is suitable for

Constructing recombinant DNA by joining with vectors

Which one of the following represents a palindromic sequence in DNA?

5' - GAATTC - 3' 3' - CTTAAG - 5'

5' - CCAATG - 3' 3' - GAATCC - 5'

5' - CATTAG - 3' 3' - GATAAC - 5'

5' - GATACC - 3' 3' - CCTAAG - 5'

E M B I B E

Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 24

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IMPORTANT

Earn 100

Study the given diagram below of the stirred tank fermenter. What is the function of part labeled A?

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Important Questions on Biotechnology - Principles and Processes

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 25

An advantage of using yeast rather than bacteria as recipient cells for the recombinant DNA of eukaryotes is that yeast can

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 26

Which of the following is an incorrect statement?

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 27

Which of the following is incorrect with respect to plasmids?

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 28

In order to induce the bacterial uptake of plasmids, the bacteria are made competent by first treating with

HARD

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IMPORTANT

Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 29

If a person obtains transformants by inserting a recombinant DNA within the coding sequence of enzyme

β

-galactosidase, he will separate out recombinants from non-recombinants by which of the following observations?

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 30

Restriction endonucleases are utilised in genetic engineering as

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 31

Which of the following is an incorrect statement?

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 32

In a polymerase chain reaction, temperature required for the steps, respectively
(i) Denaturation,
(ii) Annealing and
(iii) Extension

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