
Suggest how using food processing waste materials as substrates for the production of pectinase could benefit the environment.

Important Questions on Biotechnology and Genetic Modification
Penicillium viridicatum is grown in fermenters.
What is a fermenter?




Many people in the world do not have enough vitamin A in their diet. We can make vitamin A in the body from carotene, which is found naturally in many plants. Rice has all the genes for making carotene, but some of these genes are turned off in the rice grains.
Researchers have added two genes to rice plants, which code for the production of two enzymes that enable the production of carotene in rice grains. Figure 20.1 shows how one of the genes, from a bacterium, was added.
On figure 20.1, write the letter R at one step at which restriction enzymes would be used.

Explain what is sticky ends are.


Many people in the world do not have enough vitamin A in their diet. We can make vitamin A in the body from carotene, which is found naturally in many plants. Rice has all the genes for making carotene, but some of these genes are turned off in the rice grains.
Researchers have added two genes to rice plants, which code for the production of two enzymes that enable the production of carotene in rice grains. Figure 20.1 shows how one of the genes, from a bacterium, was added.
In figure 20.1, write the letter L to indicate one step at which DNA ligase would be used.
