The enzyme used to combine two fragments of DNA is:

Important Questions on Biotechnology - Principles and Processes

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 10

The role of DNA ligase in the construction of recombinant DNA molecule is:

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 11

The specific DNA sequence where EcoRI cuts is:

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 12

Match Column I with Column II and select the correct answer from the given codes.

	Column I		Column II
$\left(\mathrm{a}\right)$	ampR gene	$\left(\mathrm{i}\right)$	Artificial plasmid
$\left(\mathrm{b}\right)$	Separation of DNA fragments	$\left(\mathrm{i}\mathrm{i}\right)$	Selectable marker
$\left(\mathrm{c}\right)$	Hindlll	$\left(\mathrm{i}\mathrm{i}\mathrm{i}\right)$	Electrophoresis
$\left(\mathrm{d}\right)$	pBR322	$\left(\mathrm{i}\mathrm{v}\right)$	Haemophilus influenzae

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 13

How are transformants selected from non-transformants?

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 14

The gene ‘rop’ present in pBR322 cloning vector, codes for:

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 15

Identify A, B, C and D in the given figure of E. coli cloning vector pBR322 and select the correct option.

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 16

Which of the following is not a vector-less method of gene transfer?

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Biology Crash Course NEET>Chapter 33 - Biotechnology - Principles and Processes>Level 2>Q 17

Read the following statements and select the correct ones.
(i) Electrophoresis is a technique used for the separation of molecules based on their size and charge.
(ii) Plasmids are extra-chromosomal, self-replicating, usually circular, double stranded DNA molecules found naturally in many bacteria and also in some yeast.
(iii) It is not advisable to use an exonuclease enzyme while producing a recombinant DNA molecule.
(iv) In EcoRI, the roman numeral I indicates that it was the first enzyme isolated from E.coli RY 13.