EASY
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The enzymes required to obtain protoplasts are

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Important Questions on Develop and use a model to illustrate the hierarchical organization of interacting systems that provide specific functions within multicellular organisms.

HARD

Study the following

(I) Km value Affinity of the enzyme Inverse measure enzyme affinity
(II) Hydrolases P-O bonds Linking 2 compounds
(III) Transferases Transfer of a group Inhibitor of reaction
(IV) Emil Fisher Lock and key hypothesis Formation of ES complex

The correct combinations are