HARD
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The first human hormone prepared through the genetic engineering is Humulin.

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Important Questions on Biotechnology and its Applications

EASY
The two polypeptides of human insulin are linked together by:
EASY

Identify the correct sequence of steps that are followed in gene therapy.
A Altered virus is mixed with cells from patient
B. Therapeutic human gene is inserted into the virus (vector)
C. Cells are collected from the patients
D. Cells from the patient become genetically altered
E. Reintroduction of genetically altered cells into patient
F. Suitable virus (vector) is selected and altered
G The genetically altered cells produce the desired proteins or hormones

MEDIUM

Match the following 

   List - I     List - II
(a) First clinical gene therapy (i) DNA Amplification
(b) Transfection (ii) Introduction of genetically modified DNA into a eukaryotic cell
(c) Synthetic oligodeoxynucleotides (iii) Adenosine deaminase
(d) PCR (iv) Silencing genes

The correct answer is

MEDIUM
Which is correct regarding genetically engineered insulin produced using E. coli?
EASY

Fill in the blanks with the correct options given in the bracket.

_____ is the replacement of normal gene in place of the gene. (Gene therapy, Cloning, Transgenesis, All of these)

EASY
The step that proved to be the main challenging obstacle in the production of human insulin by genetic engineering is:
EASY
Peptide A and peptide B is linked by how many disulphide linkage between their proinsulin?
EASY
The _____ is the main lymphoid organ where all blood cells including lymphocytes are produced.
MEDIUM
The full form of ADA is _____ (adenosine deaminase/adenine deaminase).
MEDIUM

Fill in the blanks with the correct options given in the bracket.

_____ is the first hormone synthesise artificially. (Thyroxine, Testosterone, Insulin, Estrogen)

EASY
By the technique _____ the faulty gene is replaced by normally active gene.
EASY
The first clinical gene therapy was given in 1990 to a 4-year old girl with which of the following enzyme deficiency?
HARD

Fill in the blank with the correct word given in the bracket.

Emphysema is treated by giving protein alpha 1 _____ (antitrypsin/pepsin).

HARD
Severe combined immunodeficiency (SCID) is expressed due to the absence of an enzyme _____.
MEDIUM

Fill in the blank with the correct word given in the bracket.

Two polypeptide chains in insulin is connected by _____ (hydrogen/disulphide) bonds.

HARD
Recombinant insulin in the trade name of HUMULIN is manufactured by _____.
HARD
A forensic analysis of DNA for establishing the identity of a person is known as _____.