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The first human hormone prepared through the genetic engineering is Humulin.

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Important Questions on Biotechnology and its Applications

EASY
The two polypeptides of human insulin are linked together by:
EASY

Identify the correct sequence of steps that are followed in gene therapy.
A Altered virus is mixed with cells from patient
B. Therapeutic human gene is inserted into the virus (vector)
C. Cells are collected from the patients
D. Cells from the patient become genetically altered
E. Reintroduction of genetically altered cells into patient
F. Suitable virus (vector) is selected and altered
G The genetically altered cells produce the desired proteins or hormones

MEDIUM

Match the following 

   List - I     List - II
(a) First clinical gene therapy (i) DNA Amplification
(b) Transfection (ii) Introduction of genetically modified DNA into a eukaryotic cell
(c) Synthetic oligodeoxynucleotides (iii) Adenosine deaminase
(d) PCR (iv) Silencing genes

The correct answer is

MEDIUM
Mention under what conditions is a permanent cure of the ADA deficiency disease possible.
EASY
The step that proved to be the main challenging obstacle in the production of human insulin by genetic engineering is:
EASY
ADA is an enzyme which is deficient in a genetic disorder SCID. What is the full form of ADA?    
MEDIUM
What is gene therapy? Illustrate using the example of adenosine deaminase (ADA) deficiency.
MEDIUM
Which is correct regarding genetically engineered insulin produced using E. coli?
MEDIUM
During the processing of ‘proinsulin’ into mature ‘insulin’
EASY
The first clinical gene therapy was given in 1990 to a 4-year old girl with which of the following enzyme deficiency?
EASY
The first clinical gene therapy was done for the treatment of
MEDIUM
Explain enzyme-replacement therapy to treat adenosine deaminase deficiency. Mention two disadvantages of this procedure.
MEDIUM
Human insulin is being commercially produced from a transgenic species of
MEDIUM
Enumerate the steps involved in ADA deficiency gene therapy.