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The full name of GMO is Genetics modified organism.

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Important Questions on Biotechnology

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Suppose DNA samples collected for DNA fingerprinting analysis are less than the required quantity. Which of the following techniques is helpful to make the samples sufficient for above analysis?
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Select the correct steps involved in amplification of gene
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Sequence of strategies involved for the isolation of gene of interest from a plant cell during the process of rDNA technology
A) Dissolution of biological membranes
B) Enzymatic digestion of cell wall
C) Precipitation of DNA
D) Removal of DNA by spooling
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The DNA fragments separated on an agarose gel can be visualised after staining with:
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Assertion (A): The fragments of DNA generated by the action of restriction endonucleases are separated by polyacrylamide gel electrophoresis.

Reason (R): The separated DNA fragments are visualised as bright orange coloured bands after staining with Ethidium Bromide followed by exposure to UV - radiation.

Which of the following is true?

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What is the following is correct for the movement of DNA fragments on agarose gel during gel electrophoresis?
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DNA's precipitation out of a mixture of biomolecules can be achieved by treatment with:
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The core techniques enabled modern biotechnology
A) Change the phenotype of host-organism by genetic engineering
B) Chemical engineering process of vaccine production by tissue culture
C) Manufacture of specific products from eukaryotic cells in bioreactors
D) Altering the chemistry of genetic material by tissue culture
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Which ONE of the following is NOT essential for Polymerase Chain Reaction (PCR)?
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Which one among the following primer pairs would amplify the fragment of DNA given below?

5'-CTAGTCGTCGAT-N300-GACTGAGCTGAGCTG-3'

3'·GATCAGCAGCTA-(N)300-CTGACTCGACTCGAC-5'

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Identify the labels M and N in the following Agarose gel electrophoresis representation.

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In which of the following steps in DNA fingerprinting technique are labelled VNTR probes used?
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Which ONE of the following combinations of molecular masses of polypeptides are obtained from a purified human IgM when gel electrophoresis (SDS-PAGE) under reducing conditions?
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In a crime investigation, the investigating officer collects different biological samples from the crime spot for DNA Finger-Printing Analysis. Which of the following samples is not helpful in the analysis?
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In gel electrophoresis, separated DNA fragments can be visualized with the help of
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If the initial number of templets DNA molecules in a PCR reaction is 1000, the number of product DNA molecules at the end of 20 cycles will be closest to
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Which of the following is not required for any of the techniques of DNA fingerprinting available at present?