EASY
12th CBSE
IMPORTANT
Earn 100

The most important feature in a plasmid to serve as a vector in gene cloning experiment is:
(a)origin of replication (ori)
(b)presence of a selectable marker
(c)presence of sites for restriction endonuclease
(d)its size

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Important Questions on Biotechnology: Principles and Processes
MEDIUM
12th CBSE
IMPORTANT
While isolating DNA from bacteria, which of the following enzymes is not used?

MEDIUM
12th CBSE
IMPORTANT
Which of the following has contributed in popularising the PCR (polymerase chain reactions) technique?

MEDIUM
12th CBSE
IMPORTANT
An antibiotic resistance gene in a vector usually helps in the selection of
Note: there is an ambiguity in one of the option. Hence, modified.

MEDIUM
12th CBSE
IMPORTANT
Significance of 'heat shock' method in bacterial transformation is to facilitate

MEDIUM
12th CBSE
IMPORTANT
The role of DNA ligase in the construction of a recombinant DNA molecule is

MEDIUM
12th CBSE
IMPORTANT
Which of the following is not a source of restriction endonuclease?

MEDIUM
12th CBSE
IMPORTANT
Which of the following steps are catalysed by Taq polymerase in a PCR reaction?

HARD
12th CBSE
IMPORTANT
A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be
